C57BL/6NTac-Gclctm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05085
International strain nameC57BL/6NTac-Gclctm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0051_3_D07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolGclctm1a(EUCOMM)Wtsi
Gene/Transgene symbolGclc
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0051_3_D07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased circulating calcium level / IMPC
  • hyperplasia / IMPC
  • lipid deposition / IMPC
IMPC phenotypes (gene matching)
  • lipid deposition / IMPC
  • hyperplasia / IMPC
  • decreased circulating calcium level / IMPC
MGI phenotypes (gene matching)
  • decreased cell proliferation / MGI
  • abnormal liver physiology / MGI
  • abnormal embryo development / MGI
  • absent mesoderm / MGI
  • failure to gastrulate / MGI
  • embryonic growth arrest / MGI
  • no abnormal phenotype detected / MGI
  • abnormal embryonic-extraembryonic boundary morphology / MGI
  • embryonic growth retardation / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality between implantation and somite formation, complete penetrance / MGI
  • increased embryonic tissue cell apoptosis / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Oxidative Stress Inhibits Healthy Adipose Expansion Through Suppression of SREBF1-Mediated Lipogenic Pathway.;Okuno Yosuke, Fukuhara Atsunori, Hashimoto Erika, Kobayashi Hironori, Kobayashi Sachiko, Otsuki Michio, Shimomura Iichiro, ;2018;Diabetes;67;1113-1127; 29618580
  • Neuronal glutathione loss leads to neurodegeneration involving gasdermin activation.;Hashimoto Shoko, Matsuba Yukio, Takahashi Mika, Kamano Naoko, Watamura Naoto, Sasaguri Hiroki, Takado Yuhei, Yoshihara Yoshihiro, Saito Takashi, Saido Takaomi C, ;2023;Scientific reports;13;1109; 36670138
  • Neuronal glutathione depletion elevates the Aβ42/Aβ40 ratio and tau aggregation in Alzheimer's disease mice.;Hashim Khairun Nisa Binti, Matsuba Yukio, Takahashi Mika, Kamano Naoko, Tooyama Ikuo, Saido Takaomi C, Hashimoto Shoko, ;2024;FEBS letters;598;1576-1590; 38789405

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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