B6Brd;B6N-Tyrc-Brd Rnaseh2btm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05078
International strain nameB6Brd;B6N-Tyrc-Brd Rnaseh2btm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0087_4_A02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolRnaseh2btm1a(EUCOMM)Wtsi
Gene/Transgene symbolRnaseh2b
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0087_4_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (allele matching)
  • decreased cell proliferation / MGI
  • liver hypoplasia / MGI
  • abnormal blood vessel morphology / MGI
  • decreased embryo size / MGI
  • edema / MGI
  • thymus hypoplasia / MGI
  • hemorrhage / MGI
  • embryonic growth retardation / MGI
  • decreased birth body size / MGI
  • preweaning lethality / MGI
  • perinatal lethality, complete penetrance / MGI
  • decreased fibroblast proliferation / MGI
MGI phenotypes (gene matching)
  • decreased cell proliferation / MGI
  • abnormal cell morphology / MGI
  • liver hypoplasia / MGI
  • abnormal blood vessel morphology / MGI
  • decreased embryo size / MGI
  • abnormal allantois morphology / MGI
  • edema / MGI
  • thymus hypoplasia / MGI
  • hemorrhage / MGI
  • embryonic growth retardation / MGI
  • caudal body truncation / MGI
  • decreased birth body size / MGI
  • impaired somite development / MGI
  • preweaning lethality / MGI
  • perinatal lethality, complete penetrance / MGI
  • decreased fibroblast proliferation / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • RNase H2 Loss in Murine Astrocytes Results in Cellular Defects Reminiscent of Nucleic Acid-Mediated Autoinflammation.;Bartsch Kareen, Damme Markus, Regen Tommy, Becker Lore, Garrett Lillian, Hölter Sabine M, Knittler Katharina, Borowski Christopher, Waisman Ari, Glatzel Markus, Fuchs Helmut, Gailus-Durner Valerie, Hrabe de Angelis Martin, Rabe Björn, ;2018;Frontiers in immunology;9;587; 29662492
  • Genome instability independent of type I interferon signaling drives neuropathology caused by impaired ribonucleotide excision repair.;Aditi null, Downing Susanna M, Schreiner Patrick A, Kwak Young Don, Li Yang, Shaw Timothy I, Russell Helen R, McKinnon Peter J, ;2021;Neuron;109;3962-3979.e6; 34655526

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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