B6;CBA-Tg(TNF)197Gkl/Flmg

Status	Available to order
EMMA ID	EM:04975
International strain name	B6;CBA-Tg(TNF)197Gkl/Flmg
Alternative name	B6;CBA-Tg(TNF-HBB)197Gkl/Flmg, B6;CBA-Tg(huTNF)197Gkl/Flmg
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(TNF)197Gkl
Gene/Transgene symbol	Tg(TNF)197Gkl

Information from provider

Provider	George Kollias
Provider affiliation	B.S.R.C.
Genetic information	The human TNF/beta-globin (TNF-globin) recombinant gene construct was prepared in the pBluescript vector as follows: The 2.8 kb EcoRI fragment which contains the entire coding region of the human TNF gene was ligated to the 0.77 kb EcoRI-Sall fragment which contains the 3'-untranslated region and polyadenylation site of the human beta-globin gene (Kollias et al., 1987) and excised for microinjection as a BamHI -Sall fragment.
Phenotypic information	Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.
References	Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.;Keffer J, Probert L, Cazlaris H, Georgopoulos S, Kaslaris E, Kioussis D, Kollias G, ;1991;The EMBO journal;10;4025-31; 1721867 Combination of subtherapeutic anti-TNF dose with dasatinib restores clinical and molecular arthritogenic profiles better than standard anti-TNF treatment.;Ntari Lydia, Nikolaou Christoforos, Kranidioti Ksanthi, Papadopoulou Dimitra, Christodoulou-Vafeiadou Eleni, Chouvardas Panagiotis, Meier Florian, Geka Christina, Denis Maria C, Karagianni Niki, Kollias George, ;2021;Journal of translational medicine;19;165; 33892739
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous 0, wild-type 0

Disease and phenotype information

MGI allele-associated human disease models

rheumatoid arthritis / DOID:7148

Literature references

Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.;Keffer J, Probert L, Cazlaris H, Georgopoulos S, Kaslaris E, Kioussis D, Kollias G, ;1991;The EMBO journal;10;4025-31; 1721867
Combination of subtherapeutic anti-TNF dose with dasatinib restores clinical and molecular arthritogenic profiles better than standard anti-TNF treatment.;Ntari Lydia, Nikolaou Christoforos, Kranidioti Ksanthi, Papadopoulou Dimitra, Christodoulou-Vafeiadou Eleni, Chouvardas Panagiotis, Meier Florian, Geka Christina, Denis Maria C, Karagianni Niki, Kollias George, ;2021;Journal of translational medicine;19;165; 33892739

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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