C57BL/6N-Smoc1^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:04902
Citation information	RRID:IMSR_EM:04902 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Smoc1^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0348_1_C03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Smoc1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Smoc1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0348_1_C03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac USA, and maintained on this background thereafter.

Disease and phenotype information

MGI allele-associated human disease models

microphthalmia with limb anomalies / DOID:0060861

Orphanet associated rare diseases, based on orthologous gene matching

Microphthalmia with limb anomalies / Orphanet_1106

IMPC phenotypes (allele matching)

abnormal coat/hair pigmentation / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
abnormal snout morphology / IMPC
abnormal head size / IMPC
syndactyly / IMPC
decreased lymphocyte cell number / IMPC
increased neutrophil cell number / IMPC
increased basophil cell number / IMPC
increased heart weight / IMPC
abnormal cranium morphology / IMPC
mydriasis / IMPC
abnormal retina vasculature morphology / IMPC
short tibia / IMPC
decreased erythrocyte cell number / IMPC
increased red blood cell distribution width / IMPC
abnormal gait / IMPC
abnormal digit morphology / IMPC
impaired pupillary reflex / IMPC
abnormal retina morphology / IMPC
narrow eye opening / IMPC
abnormal retina blood vessel morphology / IMPC
abnormal optic disk morphology / IMPC
decreased circulating iron level / IMPC
abnormal maxilla morphology / IMPC
increased large unstained cell number / IMPC
abnormal coat/hair pigmentation / IMPC
abnormal pelvic girdle bone morphology / IMPC
abnormal lens morphology / IMPC

MGI phenotypes (allele matching)

cleft palate / MGI
syndactyly / MGI
decreased body size / MGI
decreased embryo size / MGI
abnormal retinal pigment epithelium morphology / MGI
absent fibula / MGI
optic nerve hypoplasia / MGI
iris coloboma / MGI
retina coloboma / MGI
neonatal lethality, complete penetrance / MGI

MGI phenotypes (gene matching)

cleft palate / MGI
abnormal hindlimb morphology / MGI
syndactyly / MGI
synostosis / MGI
interdigital webbing / MGI
abnormal autopod morphology / MGI
clubfoot / MGI
decreased body weight / MGI
decreased body size / MGI
microphthalmia / MGI
abnormal retina morphology / MGI
retinal degeneration / MGI
absent optic nerve / MGI
decreased embryo size / MGI
postnatal growth retardation / MGI
short fibula / MGI
abnormal joint morphology / MGI
high palate / MGI
bowed tibia / MGI
fused metatarsal bones / MGI
abnormal retinal pigment epithelium morphology / MGI
absent fibula / MGI
optic nerve hypoplasia / MGI
decreased retinal ganglion cell number / MGI
abnormal interdigital cell death / MGI
iris coloboma / MGI
retina coloboma / MGI
abnormal pollex morphology / MGI
postnatal lethality, complete penetrance / MGI
neonatal lethality, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Smoc1tm1a(EUCOMM)Wtsi/H