IMPC phenotypes (gene matching)
B6.129(SJL)-Slc6a3tm1(cre)Xz/Cnrm
| Status | Available to order |
| EMMA ID | EM:04893 |
| International strain name | B6.129(SJL)-Slc6a3tm1(cre)Xz/Cnrm |
| Alternative name | DAT-cre |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Slc6a3tm1(cre)Xz |
| Gene/Transgene symbol | Slc6a3 |
Information from provider
| Provider | Rene Hen |
| Provider affiliation | Columbia University |
| Genetic information | A targeting vector was constructed by inserting a cassette with the cre recombinase coding sequence, a nuclear translocation signal, and a PGK-neo gene flanked by FRT sites, into the 5'-UTR of the Slc6a3 (or dopamine transporter, Dat) gene. After homologous recombination in ES cells, mice with cre recombinase expressed from the Slc6a3 gene were produced. Knock-in mice were crossed with transgenic flp-deleter mice to remove the neo marker. A line was established where the neo was removed and the flp recombinase transgene was absent. Cre recombinase expression is detected in the ventral tegmental area (VAT) and substantia nigra zona compacta (SNc). |
| Phenotypic information | No abnormal phenotype detected. |
| Breeding history | The line was backcrossed in C57BL/6 for 10 generations and it is currently maintained by siblings breedings. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Infantile dystonia-parkinsonism / Orphanet_238455
MGI phenotypes (allele matching)
- hyperactivity / MGI
MGI phenotypes (gene matching)
- decreased body weight / MGI
- decreased body size / MGI
- abnormal maternal nurturing / MGI
- hyperactivity / MGI
- impaired coordination / MGI
- short stride length / MGI
- increased stereotypic behavior / MGI
- increased exploration in new environment / MGI
- abnormal grooming behavior / MGI
- abnormal spatial learning / MGI
- postnatal growth retardation / MGI
- abnormal pituitary secretion / MGI
- increased dopamine level / MGI
- premature death / MGI
- no abnormal phenotype detected / MGI
- abnormal CNS synaptic transmission / MGI
- disproportionate dwarf / MGI
- increased vertical activity / MGI
- decreased vertical activity / MGI
- increased thigmotaxis / MGI
- abnormal inhibitory postsynaptic currents / MGI
- enhanced long term potentiation / MGI
- abnormal dopaminergic neuron morphology / MGI
- loss of dopaminergic neurons / MGI
- abnormal locomotor activation / MGI
- hypopituitarism / MGI
- abnormal neurotransmitter uptake / MGI
- abnormal response to novel object / MGI
- abnormal response to novel odor / MGI
- nervous system phenotype / MGI
- decreased neurotransmitter release / MGI
- abnormal adenohypophysis morphology / MGI
- decreased length of long bones / MGI
- abnormal neuron physiology / MGI
- abnormal behavior / MGI
- small pituitary gland / MGI
- growth/size/body region phenotype / MGI
- behavior/neurological phenotype / MGI
- reproductive system phenotype / MGI
- taste/olfaction phenotype / MGI
- abnormal action potential / MGI
- decreased dopamine level / MGI
- abnormal pituitary gland physiology / MGI
- decreased somatotroph cell number / MGI
- decreased lactotroph cell number / MGI
- adenohypophysis hypoplasia / MGI
- pituitary intermediate lobe hypoplasia / MGI
- abnormal physiological response to xenobiotic / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- abnormal miniature inhibitory postsynaptic currents / MGI
- impaired conditioned place preference behavior / MGI
- abnormal behavioral response to xenobiotic / MGI
- increased serotonin level / MGI
- abnormal synaptic dopamine release / MGI
- lactation failure / MGI
- mortality/aging / MGI
- lethality, incomplete penetrance / MGI
- decreased dopaminergic neuron number / MGI
- decreased susceptibility to dopaminergic neuron neurotoxicity / MGI
Literature references
- Targeted gene expression in dopamine and serotonin neurons of the mouse brain.;Zhuang Xiaoxi, Masson Justine, Gingrich Jay A, Rayport Stephen, Hen René, ;2005;Journal of neuroscience methods;143;27-32; 15763133