B6.129S2-Cdkn1a^tm1Tyj/RvoCnbc

Status	Available to order
EMMA ID	EM:04869
International strain name	B6.129S2-Cdkn1a^tm1Tyj/RvoCnbc
Alternative name	B6-Cdkn1atm1Tyj/JRvO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cdkn1a^tm1Tyj
Gene/Transgene symbol	Cdkn1a

Information from provider

Provider	Ronald van Os
Provider affiliation	Molecular Genetics, University Medical Center Groningen
Genetic information	B6;129S2-Cdkn1a^tm1Tyj/J were backcrossed for 10 generations on a C57BL/6 background resulting in a new strain: B6-Cdkn1a^tm1Tyj/JRvO.
Phenotypic information	In contrast to the same mutation on a 129 background, the C57BL/6 background has no phenotype in hematopoietic stem cells. Overall, the mice have a higher sensitivity to ionizing radiation.
Breeding history	B6;129S2-Cdkn1a^tm1Tyj/J were backcrossed for 10 generations on a C57BL/6 background resulting in a new strain: B6-Cdkn1a^tm1Tyj/JRvO. Currently, mice are bred as one homozygous male with one homozygous female.
References	A Limited role for p21Cip1/Waf1 in maintaining normal hematopoietic stem cell functioning.;van Os Ronald, Kamminga Leonie M, Ausema Albertina, Bystrykh Leonid V, Draijer Deanna P, van Pelt Kyrjon, Dontje Bert, de Haan Gerald, ;2007;Stem cells (Dayton, Ohio);25;836-43; 17170062 Radiation-induced cell cycle arrest compromised by p21 deficiency.;Brugarolas J, Chandrasekaran C, Gordon J I, Beach D, Jacks T, Hannon G J, ;1995;Nature;377;552-7; 7566157
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous C57BL/6, homozygous C57BL/6
Stage of embryos	8-cell

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal heart morphology / IMPC
abnormal testis morphology / IMPC
abnormal epididymis morphology / IMPC
enlarged spleen / IMPC
enlarged epididymis / IMPC
small testis / IMPC
enlarged heart / IMPC
abnormal spleen morphology / IMPC

MGI phenotypes (allele matching)

increased malignant tumor incidence / MGI
increased incidence of tumors by ionizing radiation induction / MGI
decreased monocyte cell number / MGI
abnormal bone marrow cell morphology/development / MGI
decreased susceptibility to induced arthritis / MGI
impaired macrophage chemotaxis / MGI
hearing/vestibular/ear phenotype / MGI
cardiovascular system phenotype / MGI
increased brown adipose tissue amount / MGI
increased cell proliferation / MGI
increased body weight / MGI
decreased body weight / MGI
increased brain weight / MGI
altered response to CNS ischemic injury / MGI
increased kidney weight / MGI
increased hematopoietic stem cell number / MGI
decreased seminal vesicle weight / MGI
abnormal neuronal precursor proliferation / MGI
decreased prostate gland weight / MGI
increased neuronal precursor cell number / MGI
increased circulating cholesterol level / MGI
abnormal DNA replication / MGI
increased cellular sensitivity to ultraviolet irradiation / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased neuron number / MGI
increased white fat cell number / MGI
increased inguinal fat pad weight / MGI
increased parametrial fat pad weight / MGI
abnormal prostate gland dorsolateral lobe morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI
abnormal hematopoietic stem cell physiology / MGI
abnormal junctional epithelium morphology / MGI
abnormal keratinocyte physiology / MGI
increased squamous cell carcinoma incidence / MGI
abnormal skin physiology / MGI
abnormal cell physiology / MGI
delayed cellular replicative senescence / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
enlarged lymph nodes / MGI
decreased sensitivity to skin irradiation / MGI
increased cellular sensitivity to gamma-irradiation / MGI
increased tumor incidence / MGI
increased B cell derived lymphoma incidence / MGI
increased T cell derived lymphoma incidence / MGI
increased carcinoma incidence / MGI
premature death / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
glomerulonephritis / MGI
increased urine protein level / MGI
decreased mortality induced by ionizing radiation / MGI
kidney failure / MGI
increased hemangiosarcoma incidence / MGI
abnormal thymus physiology / MGI
increased incidence of tumors by chemical induction / MGI
decreased incidence of tumors by ionizing radiation induction / MGI
increased anti-double stranded DNA antibody level / MGI
increased anti-nuclear antigen antibody level / MGI
increased anti-histone antibody level / MGI
increased spleen weight / MGI
abnormal self tolerance / MGI
increased B cell number / MGI
increased T cell proliferation / MGI
immune system phenotype / MGI
increased memory T cell number / MGI
lymph node hyperplasia / MGI
increased IgG1 level / MGI
enlarged cervical lymph nodes / MGI
increased lung carcinoma incidence / MGI
increased histiocytic sarcoma incidence / MGI
increased Leydig cell tumor incidence / MGI
increased urinary system tumor incidence / MGI
increased skin tumor incidence / MGI
increased sebaceous gland adenoma incidence / MGI
increased metastatic potential / MGI

MGI phenotypes (gene matching)

increased brown adipose tissue amount / MGI
decreased monocyte cell number / MGI
increased cell proliferation / MGI
abnormal awl hair morphology / MGI
abnormal auchene hair morphology / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
enlarged lymph nodes / MGI
decreased sensitivity to skin irradiation / MGI
increased body weight / MGI
decreased body weight / MGI
increased metastatic potential / MGI
increased cellular sensitivity to gamma-irradiation / MGI
increased malignant tumor incidence / MGI
abnormal tumor incidence / MGI
increased tumor incidence / MGI
increased B cell derived lymphoma incidence / MGI
increased T cell derived lymphoma incidence / MGI
increased carcinoma incidence / MGI
premature death / MGI
increased brain weight / MGI
abnormal bone marrow cell morphology/development / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
hepatic steatosis / MGI
glomerulonephritis / MGI
increased urine protein level / MGI
decreased mortality induced by ionizing radiation / MGI
altered response to CNS ischemic injury / MGI
abnormal cell cycle / MGI
increased hepatocellular carcinoma incidence / MGI
decreased susceptibility to induced arthritis / MGI
abnormal keratinocyte physiology / MGI
kidney failure / MGI
increased hemangiosarcoma incidence / MGI
increased autoantibody level / MGI
abnormal thymus physiology / MGI
impaired macrophage chemotaxis / MGI
increased kidney weight / MGI
abnormal chromosome number / MGI
abnormal cell cycle checkpoint function / MGI
increased squamous cell carcinoma incidence / MGI
increased incidence of tumors by chemical induction / MGI
increased incidence of tumors by ionizing radiation induction / MGI
decreased incidence of tumors by ionizing radiation induction / MGI
increased anti-double stranded DNA antibody level / MGI
increased anti-single stranded DNA antibody level / MGI
increased anti-nuclear antigen antibody level / MGI
increased anti-histone antibody level / MGI
increased hematopoietic stem cell number / MGI
decreased seminal vesicle weight / MGI
abnormal neuronal precursor proliferation / MGI
increased spleen weight / MGI
decreased prostate gland weight / MGI
increased neuronal precursor cell number / MGI
abnormal self tolerance / MGI
increased B cell number / MGI
increased circulating cholesterol level / MGI
increased T cell proliferation / MGI
homeostasis/metabolism phenotype / MGI
hearing/vestibular/ear phenotype / MGI
growth/size/body region phenotype / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
abnormal skin physiology / MGI
abnormal cell physiology / MGI
delayed cellular replicative senescence / MGI
increased memory T cell number / MGI
abnormal DNA replication / MGI
lymph node hyperplasia / MGI
increased cellular sensitivity to ultraviolet irradiation / MGI
increased IgG1 level / MGI
decreased zigzag hair amount / MGI
enlarged cervical lymph nodes / MGI
increased lung carcinoma incidence / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased neuron number / MGI
increased white fat cell number / MGI
increased inguinal fat pad weight / MGI
increased parametrial fat pad weight / MGI
increased histiocytic sarcoma incidence / MGI
abnormal prostate gland dorsolateral lobe morphology / MGI
increased Leydig cell tumor incidence / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI
decreased lymphoma incidence / MGI
increased urinary system tumor incidence / MGI
increased skin tumor incidence / MGI
abnormal hematopoietic stem cell physiology / MGI
increased fibroblast proliferation / MGI
increased sebaceous gland adenoma incidence / MGI
abnormal junctional epithelium morphology / MGI

Literature references

A Limited role for p21Cip1/Waf1 in maintaining normal hematopoietic stem cell functioning.;van Os Ronald, Kamminga Leonie M, Ausema Albertina, Bystrykh Leonid V, Draijer Deanna P, van Pelt Kyrjon, Dontje Bert, de Haan Gerald, ;2007;Stem cells (Dayton, Ohio);25;836-43; 17170062
Radiation-induced cell cycle arrest compromised by p21 deficiency.;Brugarolas J, Chandrasekaran C, Gordon J I, Beach D, Jacks T, Hannon G J, ;1995;Nature;377;552-7; 7566157

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129S2-Cdkn1atm1Tyj/RvoCnbc