B6.129-Pls1tm1Fri/Cnbc

Status

Available to order

EMMA IDEM:04841
International strain nameB6.129-Pls1tm1Fri/Cnbc
Alternative namePls1 null
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPls1tm1Fri
Gene/Transgene symbolPls1

Information from provider

ProviderFrancisco Rivero
Provider affiliationHull York Medical School, University of Hull
Genetic informationConventional target (knockout) of the Pls1 locus, encoding plastin 1 (I-plastin). The first coding exon was replaced by a neomycin cassette.
Phenotypic informationNo overt gross phenotype. Normal intestinal microanatomy, but ultrastructural alterations: microvilli are shorter and constricted at their base, and their core actin bundles lack true rootlets. The composition of the microvilli themselves is apparently normal, whereas that of the terminal web is profoundly altered. The alterations result in increased fragility of the epithelium. This is seen as an increased sensitivity of the brush border to biochemical manipulations, decreased transepithelial resistance and increased sensitivity to dextran sodium sulfate-induced colitis.
Breeding historyAnimals are currently inbred homozygous on the second generation since embryo resurrection. Frozen embryos were prepared from animals backcrossed into C57BL/6 for four generations. Animals are bred in pairs and trios successfully.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd
Stage of embryos8-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Autosomal dominant non-syndromic sensorineural deafness type DFNA / Orphanet_90635
MGI phenotypes (allele matching)
  • abnormal intestinal epithelium morphology / MGI
  • abnormal hair cell mechanoelectric transduction / MGI
  • thin cochlear hair cell stereocilia / MGI
  • abnormal outer hair cell stereociliary bundle morphology / MGI
  • fused outer hair cell stereocilia / MGI
  • abnormal inner hair cell stereociliary bundle morphology / MGI
  • decreased inner hair cell stereocilia number / MGI
  • short inner hair cell stereocilia / MGI
  • homeostasis/metabolism phenotype / MGI
  • growth/size/body region phenotype / MGI
  • behavior/neurological phenotype / MGI
  • reproductive system phenotype / MGI
  • impaired hearing / MGI
  • decreased small intestinal microvillus size / MGI
  • increased susceptibility to induced colitis / MGI
  • increased enterocyte apoptosis / MGI
  • abnormal intestine physiology / MGI
  • mortality/aging / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • increased susceptibility to colitis induced morbidity/mortality / MGI
MGI phenotypes (gene matching)
  • abnormal intestinal epithelium morphology / MGI
  • abnormal hair cell mechanoelectric transduction / MGI
  • thin cochlear hair cell stereocilia / MGI
  • abnormal outer hair cell stereociliary bundle morphology / MGI
  • fused outer hair cell stereocilia / MGI
  • abnormal inner hair cell stereociliary bundle morphology / MGI
  • decreased inner hair cell stereocilia number / MGI
  • short inner hair cell stereocilia / MGI
  • homeostasis/metabolism phenotype / MGI
  • growth/size/body region phenotype / MGI
  • behavior/neurological phenotype / MGI
  • reproductive system phenotype / MGI
  • impaired hearing / MGI
  • decreased small intestinal microvillus size / MGI
  • increased susceptibility to induced colitis / MGI
  • increased enterocyte apoptosis / MGI
  • abnormal intestine physiology / MGI
  • mortality/aging / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • increased susceptibility to colitis induced morbidity/mortality / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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