IMPC phenotypes (gene matching)
- abnormal posture / IMPC
C57BL/6N-Gt(ROSA)26Sortm1(FLP1)Wtsi/WtsiIeg
| Status | Available to order |
| EMMA ID | EM:04830 |
| International strain name | C57BL/6N-Gt(ROSA)26Sortm1(FLP1)Wtsi/WtsiIeg |
| Alternative name | ROSA26 Fki |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(FLP1)Wtsi |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Haydn Prosser |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | The mouse strain is a repetition of the targeted knock-in of the FLPeR construct targeted into the Gt(ROSA)26Sor locus using C57BL/6N ES cells (JM8-F6). Original reference for the targeting construct: Widespread recombinase expression using FLPeR (flipper) mice. Farley FW, Soriano P, Steffen LS, Dymecki SM. Genesis. 2000 Nov-Dec;28(3-4):106-10. Reference for the ES cells: Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A, Skarnes WC. Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods. 2009 Jul;6(7):493-5. Epub 2009 Jun 14. |
| Phenotypic information | This is a germline deleter strain intended to delete sequence flanked by two Flp recombinase recognition (FRT) sites. |
| Breeding history | The allele was maintained as an inbred line in the C57BL/6N (Taconic) background. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal ear pigmentation / MGI
- decreased circulating LDL cholesterol level / MGI
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- decreased neutrophil cell number / MGI
- extramedullary hematopoiesis / MGI
- abnormal erythropoiesis / MGI
- abnormal myocardial fiber morphology / MGI
- abnormal cell death / MGI
- increased granulocyte number / MGI
- increased cell proliferation / MGI
- alopecia / MGI
- short snout / MGI
- abnormal digestive system morphology / MGI
- gastrointestinal hemorrhage / MGI
- increased rib number / MGI
- abnormal pulmonary artery morphology / MGI
- rectal prolapse / MGI
- abnormal renal/urinary system morphology / MGI
- abnormal mammary gland development / MGI
- abnormal spleen morphology / MGI
- enlarged spleen / MGI
- enlarged lymph nodes / MGI
- dystrophic muscle / MGI
- absent notochord / MGI
- abnormal retinal photoreceptor morphology / MGI
- abnormal neuromuscular synapse morphology / MGI
- pigmentation phenotype / MGI
- increased body weight / MGI
- decreased body weight / MGI
- decreased body size / MGI
- retinal degeneration / MGI
- disorganized retinal layers / MGI
- abnormal optic nerve morphology / MGI
- decreased anxiety-related response / MGI
- circling / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal eating behavior / MGI
- limb grasping / MGI
- increased circulating triglyceride level / MGI
- increased circulating free fatty acid level / MGI
- increased circulating HDL cholesterol level / MGI
- hyperglycemia / MGI
- anemia / MGI
- abnormal blood vessel morphology / MGI
- abnormal mesoderm development / MGI
- abnormal somite development / MGI
- decreased trophoblast giant cell number / MGI
- postnatal growth retardation / MGI
- impaired wound healing / MGI
- abnormal humoral immune response / MGI
- arrested B cell differentiation / MGI
- arrested T cell differentiation / MGI
- increased inflammatory response / MGI
- reduced fertility / MGI
- reduced female fertility / MGI
- male infertility / MGI
- decreased litter size / MGI
- respiratory distress / MGI
- abnormal pain threshold / MGI
- increased thermal nociceptive threshold / MGI
- abnormal coat/hair pigmentation / MGI
- abnormal glucose homeostasis / MGI
- increased circulating insulin level / MGI
- premature death / MGI
- abnormal tail morphology / MGI
- abnormal kidney morphology / MGI
- abnormal neural tube morphology / MGI
- no abnormal phenotype detected / MGI
- abnormal seminiferous tubule morphology / MGI
- small lymph nodes / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal bone marrow cell morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- abnormal proerythroblast morphology / MGI
- abnormal megakaryocyte morphology / MGI
- increased mean corpuscular volume / MGI
- decreased mean corpuscular volume / MGI
- increased mean platelet volume / MGI
- hepatic steatosis / MGI
- abnormal epididymis morphology / MGI
- vestigial tail / MGI
- reticulocytosis / MGI
- abnormal keratinocyte differentiation / MGI
- decreased circulating corticosterone level / MGI
- asthenozoospermia / MGI
- oligozoospermia / MGI
- abnormal renal tubule morphology / MGI
- decreased circulating insulin level / MGI
- abnormal chemical nociception / MGI
- dilated heart left ventricle / MGI
- decreased vertical activity / MGI
- abnormal notochord morphology / MGI
- abnormal ocular fundus morphology / MGI
- albuminuria / MGI
- decreased hemoglobin content / MGI
- decreased erythrocyte cell number / MGI
- no phenotypic analysis / MGI
- vertebral transformation / MGI
- abnormal lumbar vertebrae morphology / MGI
- abnormal skeletal muscle fiber morphology / MGI
- absent allantois / MGI
- hypospadia / MGI
- anal atresia / MGI
- increased erythroid progenitor cell number / MGI
- abnormal embryonic hematopoiesis / MGI
- kinked neural tube / MGI
- epididymal inflammation / MGI
- nervous system phenotype / MGI
- abnormal nervous system morphology / MGI
- kidney cysts / MGI
- pallor / MGI
- abnormal neural tube closure / MGI
- abnormal retinal inner nuclear layer morphology / MGI
- vascular smooth muscle hypoplasia / MGI
- skeletal muscle necrosis / MGI
- abnormal defecation / MGI
- abnormal heart left ventricle morphology / MGI
- abnormal circulating lipid level / MGI
- abnormal hormone level / MGI
- increased lean body mass / MGI
- abnormal rod electrophysiology / MGI
- abnormal cone electrophysiology / MGI
- caudal body truncation / MGI
- abnormal vitelline vascular remodeling / MGI
- abnormal sarcomere morphology / MGI
- increased compact bone thickness / MGI
- transmission ratio distortion / MGI
- abnormal basement membrane morphology / MGI
- short frontal bone / MGI
- short nasal bone / MGI
- increased incidence of tumors by chemical induction / MGI
- caudal vertebral transformation / MGI
- cervical vertebral transformation / MGI
- lumbar vertebral transformation / MGI
- thoracic vertebral transformation / MGI
- cervical vertebral fusion / MGI
- absent caudal vertebrae / MGI
- small sacral vertebrae / MGI
- abnormal vertebral column morphology / MGI
- notochord degeneration / MGI
- truncated notochord / MGI
- abnormal platelet morphology / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- increased hematopoietic stem cell number / MGI
- decreased testis weight / MGI
- increased energy expenditure / MGI
- decreased adiponectin level / MGI
- decreased epididymis weight / MGI
- abnormal epididymis epithelium morphology / MGI
- skin inflammation / MGI
- decreased B-1 B cell number / MGI
- increased neuronal precursor cell number / MGI
- increased osteoblast cell number / MGI
- decreased lymphocyte cell number / MGI
- decreased cardiac muscle contractility / MGI
- cachexia / MGI
- increased susceptibility to injury / MGI
- decreased circulating cholesterol level / MGI
- abnormal retinal pigment epithelium morphology / MGI
- abnormal rostral-caudal axis patterning / MGI
- glomerulosclerosis / MGI
- increased oxygen consumption / MGI
- impaired glucose tolerance / MGI
- spina bifida occulta / MGI
- decreased triglyceride level / MGI
- abnormal renal glomerulus morphology / MGI
- insulin resistance / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- growth/size/body region phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- cardiovascular system phenotype / MGI
- immune system phenotype / MGI
- reproductive system phenotype / MGI
- skeleton phenotype / MGI
- hematopoietic system phenotype / MGI
- increased circulating thyroxine level / MGI
- increased circulating triiodothyronine level / MGI
- abnormal skeleton morphology / MGI
- abnormal body temperature / MGI
- choroidal neovascularization / MGI
- abnormal eye electrophysiology / MGI
- decreased circulating glucose level / MGI
- increased mean corpuscular hemoglobin / MGI
- decreased mean corpuscular hemoglobin / MGI
- decreased angiogenesis / MGI
- increased bone mass / MGI
- abnormal cell physiology / MGI
- increased susceptibility to diet-induced obesity / MGI
- decreased susceptibility to diet-induced obesity / MGI
- decreased circulating leptin level / MGI
- increased circulating leptin level / MGI
- increased mitochondrial fission / MGI
- increased apoptosis / MGI
- abnormal tail development / MGI
- abnormal spermatid morphology / MGI
- intestine polyps / MGI
- abnormal paraxial mesoderm morphology / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- abnormal granulocyte differentiation / MGI
- abnormal monocyte differentiation / MGI
- podocyte foot process effacement / MGI
- increased germinal center B cell number / MGI
- decreased B-2 B cell number / MGI
- decreased mature B cell number / MGI
- increased megakaryocyte cell number / MGI
- abnormal bone ossification / MGI
- retinal photoreceptor degeneration / MGI
- abnormal medium spiny neuron morphology / MGI
- retinal outer nuclear layer degeneration / MGI
- increased circulating tumor necrosis factor level / MGI
- increased circulating interleukin-1 alpha level / MGI
- increased circulating interleukin-1 beta level / MGI
- increased interleukin-17 secretion / MGI
- decreased survivor rate / MGI
- increased B cell apoptosis / MGI
- increased liver iron level / MGI
- increased spleen iron level / MGI
- increased circulating iron level / MGI
- lipofuscinosis / MGI
- abnormal physiological response to xenobiotic / MGI
- decreased erythroid progenitor cell number / MGI
- anal stenosis / MGI
- hairpin sperm flagellum / MGI
- increased abdominal fat pad weight / MGI
- increased trophoblast giant cell number / MGI
- centrally nucleated skeletal muscle fibers / MGI
- skeletal muscle endomysial fibrosis / MGI
- abnormal synaptonemal complex / MGI
- abnormal mammary gland duct morphology / MGI
- decreased thymocyte apoptosis / MGI
- enlarged popliteal lymph nodes / MGI
- decreased birth body size / MGI
- impaired somite development / MGI
- increased total body fat amount / MGI
- abnormal placenta physiology / MGI
- abnormal retinal blood vessel morphology / MGI
- decreased mammary gland epithelial cell proliferation / MGI
- decreased tumor latency / MGI
- altered tumor pathology / MGI
- mortality/aging / MGI
- integument phenotype / MGI
- increased bone volume / MGI
- abnormal double-strand DNA break repair / MGI
- neonatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- prenatal lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- preweaning lethality, complete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
- abnormal anterior visceral endoderm cell migration / MGI
- increased circulating erythropoietin level / MGI
- renal tubule atrophy / MGI
- lethality, complete penetrance / MGI
- increased mitochondria number / MGI
- rectal atresia / MGI
- rectourethral fistula / MGI
- increased hematopoietic stem cell proliferation / MGI
- increased food intake / MGI
- impaired mammary gland growth during pregnancy / MGI
- decreased paraxial mesoderm size / MGI
- abnormal skeletal muscle regeneration / MGI
- testis degeneration / MGI
- preputial gland inflammation / MGI
- nervous system inclusion bodies / MGI
- decreased fatty acid beta-oxidation / MGI
- epididymis hypertrophy / MGI
- spermatocele / MGI
- epididymis fibrosis / MGI
- increased epididymal cell proliferation / MGI
- increased epididymal epithelium cell proliferation / MGI
- increased heart iron level / MGI
- increased intestinal iron level / MGI
Literature references
- Widespread recombinase expression using FLPeR (flipper) mice.;Farley F W, Soriano P, Steffen L S, Dymecki S M, ;2000;Genesis (New York, N.Y. : 2000);28;106-10; 11105051
- Agouti C57BL/6N embryonic stem cells for mouse genetic resources.;Pettitt Stephen J, Liang Qi, Rairdan Xin Y, Moran Jennifer L, Prosser Haydn M, Beier David R, Lloyd Kent C, Bradley Allan, Skarnes William C, ;2009;Nature methods;6;493-5; 19525957
- The role of sphingosine-1-phosphate transporter Spns2 in immune system function.;Nijnik Anastasia, Clare Simon, Hale Christine, Chen Jing, Raisen Claire, Mottram Lynda, Lucas Mark, Estabel Jeanne, Ryder Edward, Adissu Hibret, null null, Adams Niels C, Ramirez-Solis Ramiro, White Jacqueline K, Steel Karen P, Dougan Gordon, Hancock Robert E W, ;2012;Journal of immunology (Baltimore, Md. : 1950);189;102-11; 22664872
- Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.;Maguire Simon, Estabel Jeanne, Ingham Neil, Pearson Selina, Ryder Edward, Carragher Damian M, Walker Nicolas, null null, Bussell James, Chan Wai-In, Keane Thomas M, Adams David J, Scudamore Cheryl L, Lelliott Christopher J, Ramírez-Solis Ramiro, Karp Natasha A, Steel Karen P, White Jacqueline K, Gerdin Anna-Karin, ;2014;PloS one;9;e91807; 24642684
- Spinster homolog 2 (spns2) deficiency causes early onset progressive hearing loss.;Chen Jing, Ingham Neil, Kelly John, Jadeja Shalini, Goulding David, Pass Johanna, Mahajan Vinit B, Tsang Stephen H, Nijnik Anastasia, Jackson Ian J, White Jacqueline K, Forge Andrew, Jagger Daniel, Steel Karen P, ;2014;PLoS genetics;10;e1004688; 25356849
- Mechanotransduction is required for establishing and maintaining mature inner hair cells and regulating efferent innervation.;Corns Laura F, Johnson Stuart L, Roberts Terri, Ranatunga Kishani M, Hendry Aenea, Ceriani Federico, Safieddine Saaid, Steel Karen P, Forge Andy, Petit Christine, Furness David N, Kros Corné J, Marcotti Walter, ;2018;Nature communications;9;4015; 30275467