C57BL/6N-Atn1tm1a(EUCOMM)Wtsi/Cnrm

Status

Available to order

EMMA IDEM:04826
Citation informationRRID:IMSR_EM:04826 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Atn1tm1a(EUCOMM)Wtsi/Cnrm
Alternative nameEPD0289_1_F08
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAtn1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolAtn1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider CNR, Consiglio Nazionale delle Ricerche
Provider affiliationEMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0289_1_F08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archivingheterozygous C57BL/6NTac males, wild-type C57BL/6NTac females
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal sinus arrhythmia / IMPC
  • increased heart rate variability / IMPC
  • decreased erythrocyte cell number / IMPC
  • abnormal skin morphology / IMPC
  • decreased exploration in new environment / IMPC
  • limb grasping / IMPC
  • decreased lymphocyte cell number / IMPC
  • increased neutrophil cell number / IMPC
  • abnormal gait / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • cardiovascular system phenotype / IMPC
  • increased circulating triglyceride level / IMPC
  • decreased monocyte cell number / IMPC
  • abnormal heart morphology / IMPC
  • persistence of hyaloid vascular system / IMPC
  • cataract / IMPC
MGI phenotypes (gene matching)
  • small testis / MGI
  • decreased body weight / MGI
  • reduced male fertility / MGI
  • premature death / MGI
  • no abnormal phenotype detected / MGI
  • abnormal sperm number / MGI
  • no phenotypic analysis / MGI
  • nervous system phenotype / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • decreased food intake / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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