B6Brd;B6N-Tyrc-Brd Prkcztm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:04815
International strain nameB6Brd;B6N-Tyrc-Brd Prkcztm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0061_2_F06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPrkcztm1a(EUCOMM)Wtsi
Gene/Transgene symbolPrkcz
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0061_2_F06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased circulating LDL cholesterol level / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased lactate dehydrogenase level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased circulating creatinine level / IMPC
  • increased circulating iron level / IMPC
  • developmental and structural abnormality / IMPC
IMPC phenotypes (gene matching)
  • increased circulating aspartate transaminase level / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased circulating LDL cholesterol level / IMPC
  • developmental and structural abnormality / IMPC
  • increased circulating iron level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased circulating creatinine level / IMPC
  • increased lactate dehydrogenase level / IMPC
MGI phenotypes (allele matching)
  • increased circulating iron level / MGI
  • persistence of hyaloid vascular system / MGI
  • abnormal melanocyte morphology / MGI
  • increased circulating alkaline phosphatase level / MGI
  • increased circulating alanine transaminase level / MGI
  • increased circulating aspartate transaminase level / MGI
MGI phenotypes (gene matching)
  • abnormal Peyer's patch morphology / MGI
  • persistence of hyaloid vascular system / MGI
  • decreased anxiety-related response / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal Peyer's patch follicle morphology / MGI
  • abnormal melanocyte morphology / MGI
  • increased circulating alanine transaminase level / MGI
  • increased circulating alkaline phosphatase level / MGI
  • no phenotypic analysis / MGI
  • nervous system phenotype / MGI
  • decreased B cell number / MGI
  • increased circulating aspartate transaminase level / MGI
  • behavior/neurological phenotype / MGI
  • immune system phenotype / MGI
  • small Peyer's patches / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • increased circulating iron level / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Repression of Intestinal Stem Cell Function and Tumorigenesis through Direct Phosphorylation of β-Catenin and Yap by PKCζ.;Llado Victoria, Nakanishi Yuki, Duran Angeles, Reina-Campos Miguel, Shelton Phillip M, Linares Juan F, Yajima Tomoko, Campos Alex, Aza-Blanc Pedro, Leitges Michael, Diaz-Meco Maria T, Moscat Jorge, ;2015;Cell reports;10;740-754; 25660024
  • PKCζ Is Essential for Pancreatic β-Cell Replication During Insulin Resistance by Regulating mTOR and Cyclin-D2.;Lakshmipathi Jayalakshmi, Alvarez-Perez Juan Carlos, Rosselot Carolina, Casinelli Gabriella P, Stamateris Rachel E, Rausell-Palamos Francisco, O'Donnell Christopher P, Vasavada Rupangi C, Scott Donald K, Alonso Laura C, Garcia-Ocaña Adolfo, ;2016;Diabetes;65;1283-96; 26868297

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Availabilities

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Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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