B6Brd;B6N-Tyrc-Brd Brpf3tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:04813
International strain nameB6Brd;B6N-Tyrc-Brd Brpf3tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0027_5_E04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolBrpf3tm1a(EUCOMM)Wtsi
Gene/Transgene symbolBrpf3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0027_5_E04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased circulating glucose level / IMPC
  • decreased circulating potassium level / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
IMPC phenotypes (gene matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • decreased circulating glucose level / IMPC
  • decreased circulating potassium level / IMPC
MGI phenotypes (allele matching)
  • decreased circulating glucose level / MGI
  • decreased circulating potassium level / MGI
MGI phenotypes (gene matching)
  • homeostasis/metabolism phenotype / MGI
  • growth/size/body region phenotype / MGI
  • cellular phenotype / MGI
  • behavior/neurological phenotype / MGI
  • reproductive system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased circulating glucose level / MGI
  • decreased circulating potassium level / MGI
  • mortality/aging / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).