C57BL/6N-Bag3tm1a(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:04807
International strain nameC57BL/6N-Bag3tm1a(EUCOMM)Hmgu/H
Alternative nameHEPD0556_7_B06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolBag3tm1a(EUCOMM)Hmgu
Gene/Transgene symbolBag3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0556_7_B06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac
Breeding at archiving centreChimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • decreased blood urea nitrogen level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (gene matching)
  • hypoglycemia / MGI
  • decreased leukocyte cell number / MGI
  • decreased neutrophil cell number / MGI
  • pale liver / MGI
  • spleen hypoplasia / MGI
  • abnormal skeletal muscle morphology / MGI
  • decreased body weight / MGI
  • weight loss / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • postnatal lethality / MGI
  • premature death / MGI
  • disproportionate dwarf / MGI
  • increased circulating ketone body level / MGI
  • hepatic steatosis / MGI
  • decreased circulating triglyceride level / MGI
  • decreased circulating insulin level / MGI
  • no phenotypic analysis / MGI
  • increased cardiomyocyte apoptosis / MGI
  • decreased liver weight / MGI
  • pulmonary edema / MGI
  • abnormal Z line morphology / MGI
  • myocardial fiber degeneration / MGI
  • decreased spleen weight / MGI
  • decreased thymus weight / MGI
  • decreased lymphocyte cell number / MGI
  • decreased circulating cholesterol level / MGI
  • decreased subcutaneous adipose tissue amount / MGI
  • decreased gonadal fat pad weight / MGI
  • skeletal muscle fiber degeneration / MGI
  • postnatal lethality, complete penetrance / MGI
  • preweaning lethality, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).