IMPC phenotypes (allele matching)
B6;129S7-Cadm1tm1.2Brd/WtsiCnbc
| Status | Available to order |
| EMMA ID | EM:04806 |
| International strain name | B6;129S7-Cadm1tm1.2Brd/WtsiCnbc |
| Alternative name | Tslc1-Brdm2 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cadm1tm1.2Brd, |
| Gene/Transgene symbol | Cadm1 |
Information from provider
| Provider | Louise Van Der Weyden |
| Provider affiliation | Experimental Mouse Genetics, Wellcome Trust Sanger Institute |
| Genetic information | We have generated null allele of the Tslc1/Cadm1 gene by gene targeting. The targeted locus has loxP sites flanking exon 9 and FRT sites flanking the selection cassette. Exon 9 encodes the transmembrane domain (thus the exon 9-deleted form of the gene would not be membrane bound and would lack a functional cytoplasmic domain, and splicing of exon 8 with exon 10 would cause a shift in the reading frame). |
| Phenotypic information | Tslc1 null male mice were infertile. Tslc1 null adult testes showed that spermatogenesis had arrested at the spermatid stage, with degenerating and apoptotic spermatids sloughing off into the lumen. In adult mice, Tslc1 null round spermatids showed evidence of normal differentiation (an acrosomal cap and F-actin polarization indistinguishable from that of wild-type spermatids); however, the surviving spermatozoa were immature, malformed, found at very low levels in the epididymis, and rarely motile. Analysis of the first wave of spermatogenesis in Tslc1 null mice showed a delay in maturation by day 22 and degeneration of round spermatids by day 28. |
| Breeding history | Targeted ES cell clones (AB2.2) were transmitted through the germ line (chimaeric males bred to C57BL/6J females) and bred to homozygosity. Line maintained by interbreeding for at least 5 generations. Maintained on a mixed 129/Sv-C57BL/6 background. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Loss of TSLC1 causes male infertility due to a defect at the spermatid stage of spermatogenesis.;van der Weyden Louise, Arends Mark J, Chausiaux Oriane E, Ellis Peter J, Lange Ulrike C, Surani M Azim, Affara Nabeel, Murakami Yoshinori, Adams David J, Bradley Allan, ;2006;Molecular and cellular biology;26;3595-609; 16611999