B6.Cg-Tg(ITGAM-cre)2781Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:04788 |
| International strain name | B6.Cg-Tg(ITGAM-cre)2781Gkl/Flmg |
| Alternative name | CD11b-Cre |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(ITGAM-cre)2781Gkl |
| Gene/Transgene symbol | Tg(ITGAM-cre)2781Gkl |
Information from provider
| Provider | George Kollias |
| Provider affiliation | B.S.R.C. |
| Genetic information | For the generation of CD11b-Cre transgenic mice, a 1.7kb DNA fragment (position -1584kb - +85bp) of the human ITGAM (CD11b) promoter (Hickstein et al., 1992) was cloned by amplification, from genomic DNA derived from the human leukemic myelomonocytic cell line U937. Sense primer: 5'-CGGGGTACCGGTTCAAAGTGATTCTGC-3' introduced a KpnI restriction site, antisense primer: 5'-CCGCTCGAGTGGAAGGAGCCAGAACC-3' created a XhoI restriction site at position + 85bp of the promoter. The KpnI-XhoI fragment was cloned to respective restriction sites of the pBluescript (Stratagene) vector containing the cre recombinase gene. As a polyadenylation signal, the human growth hormone (2.2Kb) gene was used. The transgene was isolated for microinjection into fertilized eggs as a KpnI-NotI fragment. |
| Phenotypic information | No abnormal phenotype detected. This strain expresses cre recombinase under the human ITGAM (CD11b) promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, cre mediated recombination results in deletion of the targeted sequence specifically in peritoneal macrophages and microglial cells. This strain represents an effective tool for the generation of cell specific targeted mutants. |
| References |
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Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | Morula |
Literature references
- Onset and progression in inherited ALS determined by motor neurons and microglia.;Boillée Séverine, Yamanaka Koji, Lobsiger Christian S, Copeland Neal G, Jenkins Nancy A, Kassiotis George, Kollias George, Cleveland Don W, ;2006;Science (New York, N.Y.);312;1389-92; 16741123
- Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice.;Cheng Jinbo, Liao Yajin, Dong Yuan, Hu Han, Yang Nannan, Kong Xiangxi, Li Shuoshuo, Li Xiaoheng, Guo Jifeng, Qin Lixia, Yu Jiezhong, Ma Cungen, Li Jianke, Li Mingtao, Tang Beisha, Yuan Zengqiang, ;2020;Autophagy;16;2193-2205; 32003282