B6.Cg-Tg(Cr2-cre)3Cgn/Flmg

Status	Available to order
EMMA ID	EM:04787
Citation information	RRID:IMSR_EM:04787 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(Cr2-cre)3Cgn/Flmg
Alternative name	B6.CBA-CD21-Cre
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Cr2-cre)3Cgn
Gene/Transgene symbol	Tg(Cr2-cre)3Cgn

Information from provider

Provider	George Kollias
Provider affiliation	B.S.R.C.
Additional owner	Prof. Klaus Rajewsky, Immune Disease Institute, Boston, MA
Genetic information	A targeting vector, containing the cre recombinase-encoding sequence and FRT-flanked kanamycin resistance gene, was inserted into a BAC clone carrying the murine Cr2 (CD21/CD35) gene. Kanamycin was excised and CD21-cre BAC was microinjected in mouse zygotes.
Phenotypic information	None. This strain expresses cre recombinase under murine Cr2 (CD21) promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, cre-mediated recombination results in deletion of the targeted sequence specifically in B lymphocytes and follicular dendritic cells (FDCs). This strain represents an effective tool for the generation of cell-specific targeted mutants.
References	FDC-specific functions of p55TNFR and IKK2 in the development of FDC networks and of antibody responses.;Victoratos Panayiotis, Lagnel Jacques, Tzima Sotiria, Alimzhanov Marat B, Rajewsky Klaus, Pasparakis Manolis, Kollias George, ;2006;Immunity;24;65-77; 16413924 Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer.;Kraus Manfred, Alimzhanov Marat B, Rajewsky Nikolaus, Rajewsky Klaus, ;2004;Cell;117;787-800; 15186779

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece

Literature references

FDC-specific functions of p55TNFR and IKK2 in the development of FDC networks and of antibody responses.;Victoratos Panayiotis, Lagnel Jacques, Tzima Sotiria, Alimzhanov Marat B, Rajewsky Klaus, Pasparakis Manolis, Kollias George, ;2006;Immunity;24;65-77; 16413924
Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer.;Kraus Manfred, Alimzhanov Marat B, Rajewsky Nikolaus, Rajewsky Klaus, ;2004;Cell;117;787-800; 15186779

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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