FVB-Tg(tetO-TPR/MET,-EGFP)12Tcre/Cnrm
| Status | Available to order |
| EMMA ID | EM:04778 |
| International strain name | FVB-Tg(tetO-TPR/MET,-EGFP)12Tcre/Cnrm |
| Alternative name | Tpr-Met-TetO-GFP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-TPR/MET,-EGFP)12Tcre |
| Gene/Transgene symbol | Tg(tetO-TPR/MET,-EGFP)12Tcre |
Information from provider
| Provider | Tiziana Crepaldi |
| Provider affiliation | Anatomy Pharmacology and Forensic Medicine, University of Turin |
| Additional owner | Prof. Carola Ponzetto, Dept. Anatomy, Pharmacology and Forensic Medicine, Turin, Italy |
| Genetic information | In order to obtain tetracycline controlled expression of human TRP-MET oncoprotein, its cDNA was cloned into the pBI-eGFP vector (Clontech) to generate the bidirectional Tpr-Met-TetO-GFP responder. Gene expression was induced in the heart by crossing the Tpr-Met-TetO-GFP transgenic line with mice which carry a transgene consisting of the tTA driven by cardiac alpha-MHC promoter. The GFP gene facilitates detection of responder transgene on whole organ as well as in tissue sections. |
| Phenotypic information | No phenotype was observed in single transgenic mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Conditional activation of MET in differentiated skeletal muscle induces atrophy.;Crepaldi Tiziana, Bersani Francesca, Scuoppo Claudio, Accornero Paolo, Prunotto Chiara, Taulli Riccardo, Forni Paolo E, Leo Christian, Chiarle Roberto, Griffiths Jennifer, Glass David J, Ponzetto Carola, ;2007;The Journal of biological chemistry;282;6812-22; 17194700
- A mouse model for spatial and temporal expression of HGF in the heart.;Riess Ilan, Sala Valentina, Leo Christian, Demaria Marco, Gatti Stefano, Gallo Simona, Fitou Amandine, Boero Ombretta, Levi Renzo, Cuccovillo Ivan, Molla Fabiola, De Angelis Noeleen, Staszewsky Lidia, Latini Roberto, Crepaldi Tiziana, ;2011;Transgenic research;20;1203-16; 21360304
- A New Transgenic Mouse Model of Heart Failure and Cardiac Cachexia Raised by Sustained Activation of Met Tyrosine Kinase in the Heart.;Sala Valentina, Gatti Stefano, Gallo Simona, Medico Enzo, Cantarella Daniela, Cimino James, Ponzetto Antonio, Crepaldi Tiziana, ;2016;BioMed research international;2016;9549036; 27298830
- Cardiac concentric hypertrophy promoted by activated Met receptor is mitigated in vivo by inhibition of Erk1,2 signalling with Pimasertib.;Sala Valentina, Gallo Simona, Gatti Stefano, Medico Enzo, Vigna Elisa, Cantarella Daniela, Fontani Lara, Natale Massimo, Cimino James, Morello Mara, Comoglio Paolo Maria, Ponzetto Antonio, Crepaldi Tiziana, ;2016;Journal of molecular and cellular cardiology;93;84-97; 26924269