FVB-Tg(Wap-Hgf)402Mig/Cnbc
| Status | Available to order |
| EMMA ID | EM:04774 |
| International strain name | FVB-Tg(Wap-Hgf)402Mig/Cnbc |
| Alternative name | Tg(Wap-Hgf)402Mig |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Wap-Hgf)402Mig |
| Gene/Transgene symbol | Tg(Wap-Hgf)402Mig |
Information from provider
| Provider | Marta Gallego |
| Provider affiliation | Patologia Mamaria, Instituto de Salud Carlos III |
| Genetic information | Transgenic mouse strain expressing mouse Hgf under the Wap promoter. |
| Phenotypic information | WAP-HGF transgenic females developed hyperplastic ductal mammary trees and multifocal invasive mammary tumors after several pregnancies, some of which progressed to lung metastases. The WAP gene promoter, although active during estrus, is prominently induced during pregnancy and remains active throughout lactation in mammary glands. Consistent with this, continuous expression of HGF through successive pregnancies resulted in the appearance of mammary adenocarcinomas with areas of squamous metaplasia. WAP-HGF tumors expressed HGF and displayed c-Met phosphorylation. Histological and immunohistochemical analyses of sections confirmed that c-myc and Akt were activated in WAP-HGF tumors. WAP-HGF tumors presented malignancy markers like osteopontin expression and were progesterone receptor-negative. Stabilization of beta-catenin was found in areas of squamous metaplasia. |
| Breeding history | The strain was kept in an FBV genetic background for 10 generations. Sperm was frozen and cryo-preserved for 6 years, the strain was then regenerated by IVF with wild-type FVB females. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous FVB/N, wild-type FVB/N |
| Stage of embryos | 8-cell |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Targeted expression of HGF/SF in mouse mammary epithelium leads to metastatic adenosquamous carcinomas through the activation of multiple signal transduction pathways.;Gallego Marta I, Bierie Brian, Hennighausen Lothar, ;2003;Oncogene;22;8498-508; 14627990
- Hepatocyte growth factor is a lymphangiogenic factor with an indirect mechanism of action.;Cao Renhai, Björndahl Meit A, Gallego Marta I, Chen Shaohua, Religa Piotr, Hansen Anker J, Cao Yihai, ;2006;Blood;107;3531-6; 16424394