STOCK Tg(tetO-AGTR1*N111G,-lacZ)1Aco/Cnbc
| Status | Available to order |
| EMMA ID | EM:04761 |
| International strain name | STOCK Tg(tetO-AGTR1*N111G,-lacZ)1Aco/Cnbc |
| Alternative name | ZhAT1Rmut2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-AGTR1*N111G,-lacZ)1Aco |
| Gene/Transgene symbol | Tg(tetO-AGTR1*N111G,-lacZ)1Aco |
Information from provider
| Provider | Justin Ainscough |
| Provider affiliation | Central Biomedical Services, University of Leeds |
| Genetic information | A LacZ reporter gene and a constitutively active mutant form of the human angiotensin II type 1 receptor (N111G) are expressed under control of a bidirectional minimal CMV promoter linked to a tet response element (TRE). Gene expression is induced in the presence of a transactivator molecule (tTA or rtTA), and can be regulated by doxycycline. |
| Phenotypic information | No phenotype in uninduced state. Induction of hAT1Rmut expression in adult cardiomyocytes by crossing with the alphaMHC tTA transactivator mouse causes blood pressure independent cardiac hypertrophy. Effects of activation in other tissues have not been tested. |
| Breeding history | The line was crossed with transactivator mice (FVB) and subsequently backcrossed for at least four generations onto C57BL/6. Currently maintained by inbreeding. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | 8-cell |
Literature references
- Angiotensin II type-1 receptor activation in the adult heart causes blood pressure-independent hypertrophy and cardiac dysfunction.;Ainscough Justin F X, Drinkhill Mark J, Sedo Alicia, Turner Neil A, Brooke David A, Balmforth Anthony J, Ball Stephen G, ;2009;Cardiovascular research;81;592-600; 18703536