B6Dnk;B6N-Entpd1^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:04749
International strain name	B6Dnk;B6N-Entpd1^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0156_1_B01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Entpd1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Entpd1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0156_1_B01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6Dnk, wild-type C57BL/6Dnk
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive spastic paraplegia type 64 / Orphanet_401810

IMPC phenotypes (allele matching)

tremors / IMPC
increased IgE level / IMPC
decreased circulating free fatty acids level / IMPC
abnormal bone mineralization / IMPC
thrombocytopenia / IMPC
decreased circulating glycerol level / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
decreased total body fat amount / IMPC
decreased grip strength / IMPC
increased bone mineral content / IMPC

IMPC phenotypes (gene matching)

decreased grip strength / IMPC
decreased circulating glycerol level / IMPC
increased circulating chloride level / IMPC
decreased total body fat amount / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
increased bone mineral content / IMPC
thrombocytopenia / IMPC
tremors / IMPC
increased IgE level / IMPC
increased circulating sodium level / IMPC
abnormal bone mineralization / IMPC
decreased circulating free fatty acids level / IMPC

MGI phenotypes (gene matching)

abnormal blood flow velocity / MGI
impaired smooth muscle contractility / MGI
abnormal brain morphology / MGI
abnormal neutrophil physiology / MGI
increased susceptibility to ischemic brain injury / MGI
cardiac fibrosis / MGI
thrombocytopenia / MGI
liver fibrosis / MGI
renal fibrosis / MGI
abnormal vascular endothelial cell physiology / MGI
abnormal muscle electrophysiology / MGI
homeostasis/metabolism phenotype / MGI
hematopoietic system phenotype / MGI
abnormal neurotransmitter secretion / MGI
abnormal synaptic norepinephrine release / MGI
abnormal platelet physiology / MGI
increased bleeding time / MGI
pulmonary fibrosis / MGI
increased cerebral infarction size / MGI
increased neurotransmitter release / MGI
decreased platelet aggregation / MGI
spleen fibrosis / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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