IMPC phenotypes (gene matching)
B6.Cg-Hprt1tm1(Cd68-BCL2)Ple/Orl
| Status | Available to order |
| EMMA ID | EM:04748 |
| International strain name | B6.Cg-Hprt1tm1(Cd68-BCL2)Ple/Orl |
| Alternative name | Tg CD68 hBcl2 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Hprt1tm1(Cd68-BCL2)Ple |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | philippe Lesnik |
| Provider affiliation | INSERM Unit 939, Hôpital de la Pitié-Salpêtrière |
| Genetic information | Using homologous recombination in embryonic stem (ES) cells, a transgene made of the anti-apoptotic protein hBcl2 under the macrophage-specific promoter Cd68 was inserted at a defined site in the mouse genome as described previously (Farhadi et al.). Site-specific and single-copy integration of the transgene was achieved at the hypoxanthine phosphoribosyl transferase (Hprt1) locus on the X chromosome. |
| Phenotypic information | Macrophages with enhanced longevity and resistance to apoptosis. Immune system is functional. |
| Breeding history | The transgene was introduced by electroporation into BPES ES cells (hybrid C57BL/6 x 129). Targeted ES cells were injected into blastocysts, implanted into pseudopregnant females and chimeric mice generated. These chimeras transmitted the transgene through their germ line, and a number of mice bearing the transgene were obtained. Then, the transgenic mice were backcrossed 6 times to C57BL/6 genetic background. Currently continuing the backcross to C57BL/6. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- abnormal small intestine morphology / MGI
- abnormal liver morphology / MGI
- abnormal branching of the mammary ductal tree / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- tremors / MGI
- convulsive seizures / MGI
- abnormal lung morphology / MGI
- decreased body weight / MGI
- decreased anxiety-related response / MGI
- ataxia / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- short stride length / MGI
- decreased exploration in new environment / MGI
- limb grasping / MGI
- abnormal motor coordination/balance / MGI
- abnormal hematopoietic system physiology / MGI
- hyperglycemia / MGI
- anemia / MGI
- cardiac hypertrophy / MGI
- increased mammary adenocarcinoma incidence / MGI
- abnormal reflex / MGI
- seizures / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal brain morphology / MGI
- no abnormal phenotype detected / MGI
- neurodegeneration / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- hepatic steatosis / MGI
- decreased vertical activity / MGI
- increased heart weight / MGI
- increased systemic arterial blood pressure / MGI
- albuminuria / MGI
- decreased erythrocyte cell number / MGI
- increased urine protein level / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- phenotypic reversion / MGI
- abnormal dopaminergic neuron morphology / MGI
- astrocytosis / MGI
- abnormal depression-related behavior / MGI
- decreased tumor growth/size / MGI
- abnormal nervous system morphology / MGI
- abnormal cardiac muscle relaxation / MGI
- neuronal intranuclear inclusions / MGI
- abnormal myocardial fiber physiology / MGI
- abnormal Paneth cell morphology / MGI
- decreased B cell number / MGI
- decreased cardiac muscle contractility / MGI
- glomerulosclerosis / MGI
- abnormal podocyte morphology / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- taste/olfaction phenotype / MGI
- hematopoietic system phenotype / MGI
- jerky movement / MGI
- thrombocytosis / MGI
- decreased ventricle muscle contractility / MGI
- decreased mean corpuscular hemoglobin concentration / MGI
- decreased dopamine level / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- absent podocyte slit diaphragm / MGI
- podocyte foot process effacement / MGI
- increased megakaryocyte cell number / MGI
- abnormal spatial reference memory / MGI
- abnormal spatial working memory / MGI
- abnormal splenic cell ratio / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal enterocyte proliferation / MGI
- abnormal enterocyte apoptosis / MGI
- abnormal neuron differentiation / MGI
- increased mammary gland tumor incidence / MGI
- myeloid hyperplasia / MGI
- expanded mesangial matrix / MGI
- mesangial cell hyperplasia / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- decreased brain choline acetyltransferase activity / MGI
- decreased brain tyrosine 3-monooxygenase activity / MGI
- decreased vascular endothelial cell proliferation / MGI
Literature references
- Macrophage apoptosis exerts divergent effects on atherogenesis as a function of lesion stage.;Gautier Emmanuel L, Huby Thierry, Witztum Joseph L, Ouzilleau Betty, Miller Elizabeth R, Saint-Charles Flora, Aucouturier Pierre, Chapman M John, Lesnik Philippe, ;2009;Circulation;119;1795-804; 19307478
- A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes.;Farhadi Hooman F, Lepage Pierre, Forghani Reza, Friedman Hana C H, Orfali Wayel, Jasmin Luc, Miller Webb, Hudson Thomas J, Peterson Alan C, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;10214-23; 14614079