C57BL/6-Tg(Itgax-BCL2)1Ple/Orl
| Status | Available to order |
| EMMA ID | EM:04747 |
| International strain name | C57BL/6-Tg(Itgax-BCL2)1Ple/Orl |
| Alternative name | Tg CD11c-hBcl2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Itgax-BCL2)1Ple |
| Gene/Transgene symbol | Tg(Itgax-BCL2)1Ple |
Information from provider
| Provider | philippe Lesnik |
| Provider affiliation | INSERM Unit 939, Hôpital de la Pitié-Salpêtrière |
| Genetic information | hBcl-2 cDNA from pORF-hBcl-2 (Invivogen) was cloned downstream of the mouse dendritic cell-specific promoter Cd11c (Itgax) up to 821 bp from the ATG codon, amplified from a mouse BAC clone (BACPAC Resources). The linearized transgenic construct was injected into C57BL/6 fertilized female pronuclei, and progeny positive for the transgene were crossed to C57BL/6 mice. Of the three founders obtained, one animal gave germline transmission. |
| Phenotypic information | Conventional dendritic cells with enhanced longevity. Immune system is functional. The percentage of dendritic cells in the spleen is increased. |
| Breeding history | The linearized transgenic construct was injected into C57BL/6 fertilized female pronuclei, and progeny positive for the transgene were crossed to C57BL/6. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 0, wild-type C57BL/6J |
| Breeding at archiving centre | Transgenic males crossed with wild-type females. |
Literature references
- Enhanced dendritic cell survival attenuates lipopolysaccharide-induced immunosuppression and increases resistance to lethal endotoxic shock.;Gautier Emmanuel L, Huby Thierry, Saint-Charles Flora, Ouzilleau Betty, Chapman M John, Lesnik Philippe, ;2008;Journal of immunology (Baltimore, Md. : 1950);180;6941-6; 18453615
- Conventional dendritic cells at the crossroads between immunity and cholesterol homeostasis in atherosclerosis.;Gautier Emmanuel L, Huby Thierry, Saint-Charles Flora, Ouzilleau Betty, Pirault John, Deswaerte Virginie, Ginhoux Florent, Miller Elizabeth R, Witztum Joseph L, Chapman M John, Lesnik Philippe, ;2009;Circulation;119;2367-75; 19380622