C57BL/6-Tg(Itgax-BCL2)1Ple/Orl
| Status | Available to order |
| EMMA ID | EM:04747 |
| Citation information | RRID:IMSR_EM:04747 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(Itgax-BCL2)1Ple/Orl |
| Alternative name | Tg CD11c-hBcl2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Itgax-BCL2)1Ple |
| Gene/Transgene symbol | Tg(Itgax-BCL2)1Ple |
Information from provider
| Provider | philippe Lesnik |
| Provider affiliation | INSERM Unit 939, Hôpital de la Pitié-Salpêtrière |
| Genetic information | hBcl-2 cDNA from pORF-hBcl-2 (Invivogen) was cloned downstream of the mouse dendritic cell-specific promoter Cd11c (Itgax) up to 821 bp from the ATG codon, amplified from a mouse BAC clone (BACPAC Resources). The linearized transgenic construct was injected into C57BL/6 fertilized female pronuclei, and progeny positive for the transgene were crossed to C57BL/6 mice. Of the three founders obtained, one animal gave germline transmission. |
| Phenotypic information | Conventional dendritic cells with enhanced longevity. Immune system is functional. The percentage of dendritic cells in the spleen is increased. |
| Breeding history | The linearized transgenic construct was injected into C57BL/6 fertilized female pronuclei, and progeny positive for the transgene were crossed to C57BL/6. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous males, wild-type C57BL/6J females |
| Breeding at archiving centre | Transgenic males crossed with wild-type females. |
Literature references
- Enhanced dendritic cell survival attenuates lipopolysaccharide-induced immunosuppression and increases resistance to lethal endotoxic shock.;Gautier Emmanuel L, Huby Thierry, Saint-Charles Flora, Ouzilleau Betty, Chapman M John, Lesnik Philippe, ;2008;Journal of immunology (Baltimore, Md. : 1950);180;6941-6; 18453615
- Conventional dendritic cells at the crossroads between immunity and cholesterol homeostasis in atherosclerosis.;Gautier Emmanuel L, Huby Thierry, Saint-Charles Flora, Ouzilleau Betty, Pirault John, Deswaerte Virginie, Ginhoux Florent, Miller Elizabeth R, Witztum Joseph L, Chapman M John, Lesnik Philippe, ;2009;Circulation;119;2367-75; 19380622