B6.Cg-Tg(tetO-Spp1)5Gad/Orl
| Status | Available to order |
| EMMA ID | EM:04730 |
| Citation information | RRID:IMSR_EM:04730 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(tetO-Spp1)5Gad/Orl |
| Alternative name | Tet-OPN |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-Spp1)5Gad |
| Gene/Transgene symbol | Tg(tetO-Spp1)5Gad |
Information from provider
| Provider | Alain-Pierre Gadeau |
| Provider affiliation | INSERM U828 |
| Genetic information | This allele has an insertion of about 6.4Kbp including the pBI-G plasmid in which the coding sequence of Spp1/osteopontin gene was introduced. Crossing with cell specific promoter-tTA led to lineage expressing Spp1/osteopontin in the specific cell. |
| Phenotypic information | Tet-OPN mice have no phenotype since the transgene is expressed only when mice have also tTA expression. |
| Breeding history | Original background: (C57BL/6 x CBA)F2. Transgenic mice were immediately crossed with C57BL/6. The current generation is N12. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Breeding at archiving centre | Transgenic males have been crossed with wild-type females. Embryos will be transgenic or wild-type. |
Literature references
- Osteopontin expression in cardiomyocytes induces dilated cardiomyopathy.;Renault Marie-Ange, Robbesyn Fanny, Réant Patricia, Douin Victorine, Daret Danièle, Allières Cécile, Belloc Isabelle, Couffinhal Thierry, Arnal Jean-François, Klingel Karin, Desgranges Claude, Dos Santos Pierre, Charpentier Flavien, Gadeau Alain-Pierre, ;2010;Circulation. Heart failure;3;431-9; 20200330
- Estrogen-stimulated endothelial repair requires osteopontin.;Leen Laetitia Lam Shang, Filipe Cédric, Billon Audrey, Garmy-Susini Barbara, Jalvy Sandra, Robbesyn Fanny, Daret Danièle, Allières Cécile, Rittling Susan R, Werner Nikos, Nickenig Georg, Deutsch Urban, Duplàa Cécile, Dufourcq Pascale, Lenfant Françoise, Desgranges Claude, Arnal Jean-François, Gadeau Alain-Pierre, ;2008;Arteriosclerosis, thrombosis, and vascular biology;28;2131-6; 18772499