B6.Cg-Tg(tetO-Spp1)5Gad/Orl
| Status | Available to order |
| EMMA ID | EM:04730 |
| International strain name | B6.Cg-Tg(tetO-Spp1)5Gad/Orl |
| Alternative name | Tet-OPN |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-Spp1)5Gad |
| Gene/Transgene symbol | Tg(tetO-Spp1)5Gad |
Information from provider
| Provider | Alain-Pierre Gadeau |
| Provider affiliation | INSERM U828 |
| Genetic information | This allele has an insertion of about 6.4Kbp including the pBI-G plasmid in which the coding sequence of Spp1/osteopontin gene was introduced. Crossing with cell specific promoter-tTA led to lineage expressing Spp1/osteopontin in the specific cell. |
| Phenotypic information | Tet-OPN mice have no phenotype since the transgene is expressed only when mice have also tTA expression. |
| Breeding history | Original background: (C57BL/6 x CBA)F2. Transgenic mice were immediately crossed with C57BL/6. The current generation is N12. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Breeding at archiving centre | Transgenic males have been crossed with wild-type females. Embryos will be transgenic or wild-type. |
Literature references
- Osteopontin expression in cardiomyocytes induces dilated cardiomyopathy.;Renault Marie-Ange, Robbesyn Fanny, Réant Patricia, Douin Victorine, Daret Danièle, Allières Cécile, Belloc Isabelle, Couffinhal Thierry, Arnal Jean-François, Klingel Karin, Desgranges Claude, Dos Santos Pierre, Charpentier Flavien, Gadeau Alain-Pierre, ;2010;Circulation. Heart failure;3;431-9; 20200330
- Estrogen-stimulated endothelial repair requires osteopontin.;Leen Laetitia Lam Shang, Filipe Cédric, Billon Audrey, Garmy-Susini Barbara, Jalvy Sandra, Robbesyn Fanny, Daret Danièle, Allières Cécile, Rittling Susan R, Werner Nikos, Nickenig Georg, Deutsch Urban, Duplàa Cécile, Dufourcq Pascale, Lenfant Françoise, Desgranges Claude, Arnal Jean-François, Gadeau Alain-Pierre, ;2008;Arteriosclerosis, thrombosis, and vascular biology;28;2131-6; 18772499