B6Dnk;B6N-Rbfox3^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:04705
Citation information	RRID:IMSR_EM:04705 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Dnk;B6N-Rbfox3^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0543_7_B10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Rbfox3^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Rbfox3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0543_7_B10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6Dnk males
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac Den and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal tail movements / IMPC
abnormal response to new environment / IMPC
increased startle reflex / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased grip strength / IMPC

IMPC phenotypes (gene matching)

increased startle reflex / IMPC
abnormal response to new environment / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased grip strength / IMPC
abnormal tail movements / IMPC

MGI phenotypes (allele matching)

decreased anxiety-related response / MGI
hyperactivity / MGI
abnormal object recognition memory / MGI
reduced long term depression / MGI
impaired synaptic plasticity / MGI
decreased brain weight / MGI
abnormal CNS synaptic transmission / MGI
abnormal thermal nociception / MGI
increased susceptibility to pharmacologically induced seizures / MGI
abnormal excitatory postsynaptic potential / MGI
decreased paired-pulse facilitation / MGI
nervous system phenotype / MGI
abnormal miniature excitatory postsynaptic currents / MGI
decreased neuronal precursor cell number / MGI
behavior/neurological phenotype / MGI
abnormal brain white matter morphology / MGI
increased neurotransmitter release / MGI
abnormal miniature inhibitory postsynaptic currents / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI
abnormal dendritic spine morphology / MGI
abnormal neuron differentiation / MGI
abnormal hippocampus granule cell morphology / MGI
abnormal hippocampus neuron morphology / MGI
abnormal hippocampus physiology / MGI
impaired spatial learning / MGI
abnormal dentate gyrus neuron dendrite morphology / MGI
abnormal excitatory synapse morphology / MGI
decreased neuronal precursor proliferation / MGI

MGI phenotypes (gene matching)

decreased anxiety-related response / MGI
hyperactivity / MGI
abnormal object recognition memory / MGI
reduced long term depression / MGI
impaired synaptic plasticity / MGI
decreased brain weight / MGI
abnormal CNS synaptic transmission / MGI
abnormal thermal nociception / MGI
increased susceptibility to pharmacologically induced seizures / MGI
abnormal excitatory postsynaptic potential / MGI
decreased paired-pulse facilitation / MGI
nervous system phenotype / MGI
abnormal miniature excitatory postsynaptic currents / MGI
decreased neuronal precursor cell number / MGI
behavior/neurological phenotype / MGI
abnormal brain white matter morphology / MGI
increased neurotransmitter release / MGI
abnormal miniature inhibitory postsynaptic currents / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI
abnormal dendritic spine morphology / MGI
abnormal neuron differentiation / MGI
abnormal hippocampus granule cell morphology / MGI
abnormal hippocampus neuron morphology / MGI
abnormal hippocampus physiology / MGI
impaired spatial learning / MGI
abnormal dentate gyrus neuron dendrite morphology / MGI
abnormal excitatory synapse morphology / MGI
decreased neuronal precursor proliferation / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6N-Rbfox3tm1a(EUCOMM)Hmgu/H