MGI phenotypes (allele matching)
B6.Cg-Mrc2tm1Cmi Tg(CAG-EGFP)1Osb/CmiH
| Status | Available to order |
| EMMA ID | EM:04676 |
| International strain name | B6.Cg-Mrc2tm1Cmi Tg(CAG-EGFP)1Osb/CmiH |
| Alternative name | Tg(CAG-EGFP)1Osb/J Endo180ΔEx2-6/Endo180ΔEx2-6: C57BL/6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Mrc2tm1Cmi, Tg(CAG-EGFP)1Osb |
| Gene/Transgene symbol | Mrc2, Tg(CAG-EGFP)1Osb |
Information from provider
| Provider | Clare Isacke |
| Provider affiliation | Institute of Cancer Research |
| Genetic information | Exons 2-6 of the Endo180 (Mrc2) gene were replaced with a PGK-neo cassette. The deleted region includes the N-terminal cysteine-rich domain, the FNII domain and the first C-type lectin-like domain. A truncated transmembrane protein defective in collagen binding is produced but is less stable that the wild-type receptor and is barely detected in the adult animal. This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, makes all of the tissues, except for erythrocytes and hair, appear green under excitation light. |
| Phenotypic information | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. |
| Breeding history | C57BL/6 Endo180-DeltaEx2-6/Endo180-DeltaEx2-6 mice were backcrossed onto C57BL/6-Tg(CAG-EGFP)1Osb/J for 5 generations. The strain is currently maintained on C57BL/6, by mating a homozygous carrier with a C57BL/6J mouse. Expected coat color from breeding: black. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous C57BL/6J |
| Breeding at archiving centre | No breeding was carried out at the archiving centre. |
Disease and phenotype information
Literature references
- A targeted deletion in the endocytic receptor gene Endo180 results in a defect in collagen uptake.;East Lucy, McCarthy Afshan, Wienke Dirk, Sturge Justin, Ashworth Alan, Isacke Clare M, ;2003;EMBO reports;4;710-6; 12835757
- 'Green mice' as a source of ubiquitous green cells.;Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y, ;1997;FEBS letters;407;313-9; 9175875