MGI phenotypes (allele matching)
- decreased fibroblast cell migration / MGI
C.Cg-Mrc2tm1Cmi Tg(UBC-GFP)30Scha/H
| Status | Available to order |
| EMMA ID | EM:04675 |
| International strain name | C.Cg-Mrc2tm1Cmi Tg(UBC-GFP)30Scha/H |
| Alternative name | Ub-GFP Endo180ΔEx2-6/Endo180ΔEx2-6: Balb/c |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Mrc2tm1Cmi, Tg(UBC-GFP)30Scha |
| Gene/Transgene symbol | Mrc2, Tg(UBC-GFP)30Scha |
Information from provider
| Provider | Clare Isacke |
| Provider affiliation | Institute of Cancer Research |
| Genetic information | Exons 2-6 of the Endo180 (Mrc2) gene were replaced with a PGK-neo cassette. The deleted region includes the N-terminal cysteine-rich domain, the FNII domain and the first C-type lectin-like domain. A truncated transmembrane protein defective in collagen binding is produced but is less stable that the wild-type receptor and is barely detected in the adult animal. |
| Phenotypic information | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematopoietic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+/B220+ B cells or peripheral blood cells. |
| Breeding history | BALB/c Endo180-DeltaEx2-6/Endo180-DeltaEx2-6 mice were backcrossed onto BALB/c UBC-GFP for 5 generations. The strain is currently maintained on BALB/c. This strain is maintained by mating a homozygous carrier with a BALB/c mouse. Expected coat color from breeding: White. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous BALB/c |
| Breeding at archiving centre | No breeding was carried out at the archiving centre. |
Disease and phenotype information
Literature references
- A targeted deletion in the endocytic receptor gene Endo180 results in a defect in collagen uptake.;East Lucy, McCarthy Afshan, Wienke Dirk, Sturge Justin, Ashworth Alan, Isacke Clare M, ;2003;EMBO reports;4;710-6; 12835757
- Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.;Schaefer B C, Schaefer M L, Kappler J W, Marrack P, Kedl R M, ;2001;Cellular immunology;214;110-22; 12088410