IMPC phenotypes (gene matching)
- preweaning lethality, complete penetrance / IMPC
B6.129(Cg)-Criptotm1.1Gmin/Cnrm
| Status | Available to order |
| EMMA ID | EM:04657 |
| Citation information | RRID:IMSR_EM:04657 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129(Cg)-Criptotm1.1Gmin/Cnrm |
| Alternative name | Cripto F78A |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Criptotm1.1Gmin |
| Gene/Transgene symbol | Cripto |
Information from provider
| Provider | Gabriella Minchiotti |
| Provider affiliation | Institute of Genetics and Biophysics "A. Buzzati-Traverso", CNR |
| Genetic information | The mutation has been obtained by homologous recombination to introduce the amino acid substitution F78A in the Cripto (Tdgf1) gene. RI ES cells were transfected with a targeting vector that was derived from pFlox vector (Chen et al., 1998) and selected in G-418. The presence of the point mutation in the targeted cells was verified by PCR amplification and sequence analysis. Selected ES cells lines were used to generate germline chimeric mice that were subsequently bred to C57BL/6 females (Charles River Laboratories). F1 Cripto-F78Aneo heterozygotes were crossed with a Pgk-cre recombinase deleter strain to obtain the Cripto-F78A strain. For a more detailed description of the targeting vector, see D'Andrea et al. 2008, J Cell Biol. |
| Phenotypic information | Embryonic lethal at 10.5 dpc. Cripto-F78A/F78A embryos establish an A-P axis and initiate germ layer formation and gastrulation movements. The heterozygous mutants do not show any apparent phenotype, they are healthy and fertile. |
| Breeding history | Mice heterozygous for the Cripto-F78A allele are backcrossed to an inbred C57BL/6 strain. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Animals used for archiving | heterozygous C57BL/6 males, wild-type C57BL/6 females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Alobar holoprosencephaly / Orphanet_93925
- Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
- Microform holoprosencephaly / Orphanet_280200
- Septopreoptic holoprosencephaly / Orphanet_280195
- Semilobar holoprosencephaly / Orphanet_220386
- Lobar holoprosencephaly / Orphanet_93924
MGI phenotypes (gene matching)
- abnormal heart morphology / MGI
- abnormal heart development / MGI
- absent notochord / MGI
- abnormal germ layer development / MGI
- abnormal mesoderm development / MGI
- absent mesoderm / MGI
- abnormal endoderm development / MGI
- failure of primitive streak formation / MGI
- abnormal gastrulation / MGI
- abnormal embryo turning / MGI
- absent vitelline blood vessels / MGI
- embryonic growth arrest / MGI
- premature death / MGI
- abnormal developmental patterning / MGI
- abnormal embryonic tissue morphology / MGI
- abnormal extraembryonic tissue morphology / MGI
- no abnormal phenotype detected / MGI
- abnormal primitive streak formation / MGI
- abnormal chorion morphology / MGI
- abnormal cell adhesion / MGI
- abnormal embryonic neuroepithelium morphology / MGI
- absent prechordal plate / MGI
- abnormal amnion morphology / MGI
- holoprosencephaly / MGI
- cyclopia / MGI
- abnormal rostral-caudal axis patterning / MGI
- embryo phenotype / MGI
- absent somites / MGI
- abnormal mesendoderm development / MGI
- embryonic lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- abnormal visceral endoderm morphology / MGI
- abnormal visceral yolk sac blood island morphology / MGI
- abnormal neural fold morphology / MGI
- decreased fibroblast proliferation / MGI
- fused somites / MGI
- decreased midbrain size / MGI
- decreased forebrain size / MGI
- rostral body truncation / MGI
- short rostral-caudal axis / MGI
- absent anterior definitive endoderm / MGI
Literature references
- Cripto promotes A-P axis specification independently of its stimulatory effect on Nodal autoinduction.;D'Andrea Daniela, Liguori Giovanna L, Le Good J Ann, Lonardo Enza, Andersson Olov, Constam Daniel B, Persico Maria G, Minchiotti Gabriella, ;2008;The Journal of cell biology;180;597-605; 18268105