C57BL/6NTac-Uros^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:04616
International strain name	C57BL/6NTac-Uros^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0100_5_A01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Uros^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Uros
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0100_5_A01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Congenital erythropoietic porphyria / Orphanet_79277

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
increased leukocyte cell number / IMPC

MGI phenotypes (gene matching)

fragile skeleton / MGI
abnormal erythropoiesis / MGI
increased bone marrow cell number / MGI
enlarged liver / MGI
enlarged spleen / MGI
thick skin / MGI
skin photosensitivity / MGI
thick epidermis / MGI
abnormal dermal layer morphology / MGI
mixed cellular infiltration to dermis / MGI
hemolytic anemia / MGI
acanthosis / MGI
hepatic steatosis / MGI
reticulocytosis / MGI
anisocytosis / MGI
poikilocytosis / MGI
abnormal renal tubule morphology / MGI
abnormal erythrocyte physiology / MGI
increased porphyrin level / MGI
abnormal proximal convoluted tubule morphology / MGI
abnormal renal glomerulus morphology / MGI
hematopoietic system phenotype / MGI
abnormal circulating protein level / MGI
abnormal skeleton morphology / MGI
abnormal enzyme/coenzyme activity / MGI
porphyria / MGI
hypochromic microcytic anemia / MGI
increased spleen red pulp amount / MGI
increased spleen iron level / MGI
abnormal dermis papillary layer morphology / MGI
decreased birth body size / MGI
embryonic lethality before implantation, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
erythruria / MGI
erythrodontia / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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