B6Dnk;B6N-Pknox1^{Gt(EUCJ0079d10)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:04608
International strain name	B6Dnk;B6N-Pknox1^{Gt(EUCJ0079d10)Hmgu}/Ieg
Alternative name	EUCJ0079_D10
Strain type	Gene-trap
Allele/Transgene symbol	Pknox1^{Gt(EUCJ0079d10)Hmgu}
Gene/Transgene symbol	Pknox1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EUCJ0079_D10. For further details on the construction of this clone see the page at HTGT. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele. Click here for more information on EUCOMM final vectors.
References	None available

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6Dnk, wild-type C57BL/6Dnk
Stage of embryos	2-cell

IMPC phenotypes (allele matching)

IMPC phenotypes (gene matching)

MGI phenotypes (allele matching)

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal erythropoiesis / MGI
abnormal liver development / MGI
decreased body weight / MGI
abnormal eye development / MGI
small lens / MGI
anemia / MGI
absent mesoderm / MGI
failure of primitive streak formation / MGI
decreased embryo size / MGI
edema / MGI
thymus hypoplasia / MGI
hemorrhage / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
abnormal eye morphology / MGI
abnormal pluripotent precursor cell morphology / MGI
abnormal blood cell morphology/development / MGI
no phenotypic analysis / MGI
pallor / MGI
embryonic growth retardation / MGI
abnormal prenatal growth/weight/body size / MGI
decreased fetal size / MGI
increased double-negative T cell number / MGI
abnormal retinal pigment epithelium morphology / MGI
decreased percent body fat/body weight / MGI
decreased angiogenesis / MGI
abnormal retinal neuronal layer morphology / MGI
increased T cell apoptosis / MGI
decreased single-positive T cell number / MGI
decreased erythroid progenitor cell number / MGI
increased nucleated erythrocyte cell number / MGI
decreased birth body size / MGI
perinatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
abnormal visceral endoderm morphology / MGI
absent anterior visceral endoderm / MGI

Information from provider