B6Dnk;B6N-Pknox1^{Gt(EUCJ0079d10)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:04608
Citation information	RRID:IMSR_EM:04608 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Dnk;B6N-Pknox1^{Gt(EUCJ0079d10)Hmgu}/Ieg
Alternative name	EUCJ0079_D10
Strain type	Gene-trap
Allele/Transgene symbol	Pknox1^{Gt(EUCJ0079d10)Hmgu}
Gene/Transgene symbol	Pknox1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EUCJ0079_D10. For further details on the construction of this clone see the page at HTGT. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele. Click here for more information on EUCOMM final vectors.
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6Dnk males, wild-type C57BL/6Dnk females
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal urination / IMPC
decreased circulating triglyceride level / IMPC
decreased hemoglobin content / IMPC
decreased B cell number / IMPC
decreased T cell number / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
decreased mature B cell number / IMPC
decreased IgG1 level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased mean corpuscular hemoglobin concentration / IMPC
abnormal urination / IMPC
decreased IgG1 level / IMPC
decreased hemoglobin content / IMPC
decreased mature B cell number / IMPC
preweaning lethality, complete penetrance / IMPC
decreased B cell number / IMPC
decreased T cell number / IMPC
decreased circulating triglyceride level / IMPC

MGI phenotypes (allele matching)

no phenotypic analysis / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal erythropoiesis / MGI
abnormal liver development / MGI
decreased body weight / MGI
abnormal eye development / MGI
small lens / MGI
anemia / MGI
absent mesoderm / MGI
failure of primitive streak formation / MGI
decreased embryo size / MGI
edema / MGI
thymus hypoplasia / MGI
hemorrhage / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
abnormal eye morphology / MGI
abnormal pluripotent precursor cell morphology / MGI
abnormal blood cell morphology/development / MGI
no phenotypic analysis / MGI
pallor / MGI
embryonic growth retardation / MGI
abnormal prenatal growth/weight/body size / MGI
decreased fetal size / MGI
increased double-negative T cell number / MGI
abnormal retinal pigment epithelium morphology / MGI
decreased percent body fat/body weight / MGI
decreased angiogenesis / MGI
abnormal retinal neuronal layer morphology / MGI
increased T cell apoptosis / MGI
decreased single-positive T cell number / MGI
decreased erythroid progenitor cell number / MGI
increased nucleated erythrocyte cell number / MGI
decreased birth body size / MGI
perinatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
abnormal visceral endoderm morphology / MGI
absent anterior visceral endoderm / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6N-Pknox1Gt(EUCJ0079d10)Hmgu/Ieg