C57BL/6NTac-Jmjd6^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:04584
International strain name	C57BL/6NTac-Jmjd6^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0158_3_A11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Jmjd6^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Jmjd6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0158_3_A11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

increased NK cell number / IMPC
increased total body fat amount / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal motor coordination/balance / IMPC
decreased mean platelet volume / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal retina vasculature morphology / IMPC
increased total body fat amount / IMPC
abnormal lens morphology / IMPC
abnormal retina morphology / IMPC
increased startle reflex / IMPC
abnormal retina blood vessel morphology / IMPC
increased NK cell number / IMPC

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal erythropoiesis / MGI
double outlet right ventricle / MGI
abnormal intestine morphology / MGI
abnormal pulmonary artery morphology / MGI
abnormal kidney development / MGI
liver hypoplasia / MGI
abnormal thymus development / MGI
small thymus / MGI
abnormal brain development / MGI
exencephaly / MGI
abnormal lung development / MGI
anophthalmia / MGI
cyanosis / MGI
abnormal apoptosis / MGI
skin edema / MGI
impaired macrophage phagocytosis / MGI
thymus hypoplasia / MGI
respiratory failure / MGI
abnormal respiratory system morphology / MGI
decreased hemoglobin content / MGI
no phenotypic analysis / MGI
pallor / MGI
impaired macrophage chemotaxis / MGI
thin myocardium compact layer / MGI
decreased fetal size / MGI
fetal growth retardation / MGI
abnormal heart ventricle morphology / MGI
cellular phenotype / MGI
increased nucleated erythrocyte cell number / MGI
abnormal blood homeostasis / MGI
cleft secondary palate / MGI
ventricular septal defect / MGI
myocardial trabeculae hypoplasia / MGI
abnormal macrophage activation involved in immune response / MGI
neonatal lethality, complete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, incomplete penetrance / MGI
abnormal head shape / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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