IMPC phenotypes (gene matching)
B6.Cg-Bclaf1tm1Lfmp Tg(Vav1-cre)1Cgp/Ieg
| Status | Available to order |
| EMMA ID | EM:04562 |
| International strain name | B6.Cg-Bclaf1tm1Lfmp Tg(Vav1-cre)1Cgp/Ieg |
| Alternative name | Bclaf1 x Tg Vav cre C57BL/6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Bclaf1tm1Lfmp, Tg(Vav1-cre)1Cgp |
| Gene/Transgene symbol | Bclaf1, Tg(Vav1-cre)1Cgp |
Information from provider
| Provider | Rosel Blasig |
| Provider affiliation | Research Institute of Molecular Pharmacology |
| Genetic information | Promoterless reporter gene (NotI-NheI fragment of the pSA-betaGeo-lox2PGKDTA vector) containing a loxP-flanked cassette of splice acceptor, beta-galactosidase/lacZ gene and neo gene; inserted into intron between Bclaf1 exon 3 (translation start at ATG) and exon 4 by targeted mutagenesis. After breeding of heterozygous mice with transgenic Vav-cre mice on C57BL/6 genetic background the Bclaf1-/- Vav-cre+ mice are chimeras. Where the Vav promotor-driven cre recombinase is well expressed (bone marrow, spleen, blood cells, heart and lung), the Bclaf1 genotype switches from -/- via -/del to del/del (wild-type with an additional loxP site in the intron between exon 3 and 4 due to removal of loxP-flanked marker cassette). In organs without Vav promotor-driven cre recombinase expression (ear, eye, tong, optic nerve) the genotype of the mice is Bclaf1-/- Vav-cre+. |
| Phenotypic information | All Bclaf1-/- Vav-cre+ mice survive to normal life span with normal body weight. The males and females are fertile. Bclaf1-/- Vav cre-(minus) mice survive only 2-4 weeks and have 70-80% body size at birth and 35-75% body weight at 14 d age compared to littermates. If older than 2 weeks they move slowly, lay sometimes for a short time on the side, some behave sometimes like epileptically for a short time; they do not learn rearing and climbing; lower body temperature starts 4 days postnatally, lower blood glucose starts 7 days postnatally. The Bclaf1 Vav cre-(minus) mice are deposited as Bclaf1 RK BL6 on C57BL/6 background, at EMMA since 2006 (strain EM:01978). |
| Breeding history | Bclaf1 knock-out line backcrossed to C57BL/6 more than 10 generations (inbred); breeding with transgenic line expressing cre recombinase under Vav1 oncogene (Vav-cre) on C57BL/6 background. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6, wild-type C57BL/6 |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
MGI phenotypes (gene matching)
- polydactyly / MGI
- abnormal lung development / MGI
- decreased body size / MGI
- no phenotypic analysis / MGI
- increased B cell number / MGI
- decreased T cell proliferation / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- absent gastric milk in neonates / MGI
- postnatal lethality, complete penetrance / MGI
- thick lung-associated mesenchyme / MGI
- abnormal mesenchymal cell differentiation involved in lung development / MGI
Literature references
- Hematopoietic stem cell transplantation without irradiation.;Waskow Claudia, Madan Vikas, Bartels Susanne, Costa Céline, Blasig Rosel, Rodewald Hans-Reimer, ;2009;Nature methods;6;267-9; 19270698
- Essential role for Bclaf1 in lung development and immune system function.;McPherson J Peter, Sarras H, Lemmers B, Tamblyn L, Migon E, Matysiak-Zablocki E, Hakem A, Azami S Alizadeh, Cardoso R, Fish J, Sanchez O, Post M, Hakem R, ;2009;Cell death and differentiation;16;331-9; 19008920