IMPC phenotypes (gene matching)
B6.Cg-Bclaf1tm1Lfmp Tg(Nes-cre)1Kln/Ieg
| Status | Available to order |
| EMMA ID | EM:04561 |
| International strain name | B6.Cg-Bclaf1tm1Lfmp Tg(Nes-cre)1Kln/Ieg |
| Alternative name | Bclaf1 x Tg Nes cre C57BL/6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Bclaf1tm1Lfmp, Tg(Nes-cre)1Kln |
| Gene/Transgene symbol | Bclaf1, Tg(Nes-cre)1Kln |
Information from provider
| Provider | Rosel Blasig |
| Provider affiliation | Research Institute of Molecular Pharmacology |
| Genetic information | Promoterless reporter gene (NotI-NheI fragment of the vector pSA-betaGeolox2PGKDTA) containing a loxP-flanked cassette of splice acceptor, beta-galactosidase/lacZ gene and neo gene, inserted into intron between Bclaf1 exon 3 (translation start at ATG) and exon 4 by targeted mutagenesis. After breeding heterozygous mice with transgenic Nes cre mice on C57BL/6 genetic background, the Bclaf1-/- Nes cre+ mice are chimeras. Where the Nes promoter-driven cre recombinase is well expressed (brain, spinal cord and optic nerve), the Bclaf1 genotype switches from -/- via -/del to del/del (wild-type with an additional loxP site in the intron between exon 3 and 4 due to removal of loxP-flanked marker cassette). In organs without Nes promoter-driven cre recombinase expression (liver, aorta, fat tissue, thymus, spleen, lymph nodes, bone marrow) the genotype of the mice is Bclaf1-/- Nes cre+. |
| Phenotypic information | Most of the Bclaf1-/- Nes cre+ mice survive to normal life span, but show slightly reduced body weight. The males are infertile, the females have problems with breeding: they get no newborn mice (resorption of embryos?) or get only a small number of newborn mice (1-4) or sometimes giant newborn mice (1-2). Bclaf1-/- Nes cre-(minus) mice survive only 2-4 weeks and have 70-80% body size at birth and 35-75% body weight at 14 d age compared to littermates. If older than 2 weeks they move slowly, lay sometimes for a short time on the side, some behave sometimes like epileptically for a short time, they do not learn rearing and climbing. Lower body temperature starts 4 days postnatally, lower blood glucose starts 7 days postnatally. The Bclaf1 Nes cre-(minus) mice are deposited as Bclaf1 RK BL6 on C57BL/6 background at EMMA since 2006 (strain EM:01978). |
| Breeding history | Bclaf1 knock-out line backcrossed to C57BL/6 more than 10 generations (inbred); breeding with transgenic line expressing cre recombinase under nestin promoter (Nes cre) on C57BL/6 background. Homozygous males are infertile - Homozygous females have problems with breeding - Please see phenotype description! |
| References |
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| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
MGI phenotypes (gene matching)
- polydactyly / MGI
- abnormal lung development / MGI
- decreased body size / MGI
- no phenotypic analysis / MGI
- increased B cell number / MGI
- decreased T cell proliferation / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- absent gastric milk in neonates / MGI
- postnatal lethality, complete penetrance / MGI
- thick lung-associated mesenchyme / MGI
- abnormal mesenchymal cell differentiation involved in lung development / MGI
Literature references
- Hematopoietic stem cell transplantation without irradiation.;Waskow Claudia, Madan Vikas, Bartels Susanne, Costa Céline, Blasig Rosel, Rodewald Hans-Reimer, ;2009;Nature methods;6;267-9; 19270698
- Essential role for Bclaf1 in lung development and immune system function.;McPherson J Peter, Sarras H, Lemmers B, Tamblyn L, Migon E, Matysiak-Zablocki E, Hakem A, Azami S Alizadeh, Cardoso R, Fish J, Sanchez O, Post M, Hakem R, ;2009;Cell death and differentiation;16;331-9; 19008920
- Liver-derived ketone bodies are necessary for food anticipation.;Chavan Rohit, Feillet Céline, Costa Sara S Fonseca, Delorme James E, Okabe Takashi, Ripperger Jürgen A, Albrecht Urs, ;2016;Nature communications;7;10580; 26838474
- The Hepatic Monocarboxylate Transporter 1 (MCT1) Contributes to the Regulation of Food Anticipation in Mice.;Martini Tomaz, Ripperger Jürgen A, Chavan Rohit, Stumpe Michael, Netzahualcoyotzi Citlalli, Pellerin Luc, Albrecht Urs, ;2021;Frontiers in physiology;12;665476; 33935811