C57BL/6NTac-Mlh1^{tm1a(EUCOMM)Hmgu}/Cnrm

Status	Available to order
EMMA ID	EM:04517
International strain name	C57BL/6NTac-Mlh1^{tm1a(EUCOMM)Hmgu}/Cnrm
Alternative name	HEPD0522_4_D07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Mlh1^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Mlh1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	CNR, Consiglio Nazionale delle Ricerche
Provider affiliation	EMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0522_4_D07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archiving	heterozygous C57BL/6NTac, wild-type C57BL/6NTac
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Lynch syndrome / Orphanet_144
Muir-Torre syndrome / Orphanet_587
Constitutional mismatch repair deficiency syndrome / Orphanet_252202

IMPC phenotypes (gene matching)

small testis / IMPC
increased circulating alanine transaminase level / IMPC
increased spleen weight / IMPC
increased circulating aspartate transaminase level / IMPC
cataract / IMPC
male infertility / IMPC

MGI phenotypes (gene matching)

small ovary / MGI
abnormal ovarian folliculogenesis / MGI
small testis / MGI
abnormal apoptosis / MGI
abnormal humoral immune response / MGI
male infertility / MGI
female infertility / MGI
increased tumor incidence / MGI
increased B cell derived lymphoma incidence / MGI
increased T cell derived lymphoma incidence / MGI
increased carcinoma incidence / MGI
premature death / MGI
increased intestinal adenoma incidence / MGI
decreased IgE level / MGI
increased IgM level / MGI
decreased corpora lutea number / MGI
oligozoospermia / MGI
increased intestinal adenocarcinoma incidence / MGI
increased squamous cell carcinoma incidence / MGI
increased basal cell carcinoma incidence / MGI
azoospermia / MGI
abnormal female meiosis / MGI
abnormal male meiosis / MGI
arrest of male meiosis / MGI
increased lung carcinoma incidence / MGI
decreased physiological sensitivity to xenobiotic / MGI
decreased sensitivity to induced cell death / MGI
abnormal chiasmata formation / MGI
increased uterus tumor incidence / MGI
increased adenocarcinoma incidence / MGI
increased small intestine adenocarcinoma incidence / MGI
abnormal meiotic spindle morphology / MGI
increased gastrointestinal tumor incidence / MGI
increased skin tumor incidence / MGI
increased adenoma incidence / MGI
preweaning lethality, incomplete penetrance / MGI
increased lymphoma incidence / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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