B6Dnk;B6N-Sp2Gt(EUCJ0012h08)Hmgu/H

Status

Available to order

EMMA IDEM:04516
International strain nameB6Dnk;B6N-Sp2Gt(EUCJ0012h08)Hmgu/H
Alternative nameEUCJ0012h08
Strain typeGene-trap
Allele/Transgene symbolSp2Gt(EUCJ0012h08)Hmgu
Gene/Transgene symbolSp2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EUCJ0012h08. For further details on the construction of this clone see the page at HTGT. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele. Click here for more information on EUCOMM final vectors.
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous 0
Breeding at archiving centreChimeras were mated to C57BL/6Dnk and maintained on this background thereafter.

Disease and phenotype information

MGI phenotypes (allele matching)
  • no phenotypic analysis / MGI
MGI phenotypes (gene matching)
  • open neural tube / MGI
  • decreased embryo size / MGI
  • abnormal embryo turning / MGI
  • hemorrhage / MGI
  • no abnormal phenotype detected / MGI
  • no phenotypic analysis / MGI
  • embryonic growth retardation / MGI
  • decreased fetal size / MGI
  • fetal growth retardation / MGI
  • postnatal lethality, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • absent fibroblast proliferation / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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