C57BL/6-Tg(CD19-cre/ERT2)1Jsv/Cnbc

Status	Available to order
EMMA ID	EM:04499
International strain name	C57BL/6-Tg(CD19-cre/ERT2)1Jsv/Cnbc
Alternative name	LC-1- hCD19CreERT2
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CD19-cre/ERT2)1Jsv
Gene/Transgene symbol	Tg(CD19-cre/ERT2)1Jsv

Information from provider

Provider	Sjef Verbeek
Provider affiliation	Human Genetics, Leiden University Medical Center (LUMC)
Genetic information	BAC transgenic strain with 75 Kb LC-1 BAC with 6.3 Kb human CD19 promoter region driving CreERT2 (tamoxifen inducible).
Phenotypic information	B lymphocyte specific tamoxifen inducible Cre transgenic mouse strain.
Breeding history	Generated with Bruce4 ES cells (C57BL/6) and further backcrossed 3 generations to C57BL/6J. Currently maintained as homozygous by intercrossing.
References	Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector.;Boross Peter, Breukel Cor, van Loo Pieter Fokko, van der Kaa Jos, Claassens Jill W, Bujard Hermann, Schönig Kai, Verbeek J Sjef, ;2009;Genesis (New York, N.Y. : 2000);47;729-35; 19621440
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos	2-cell

Literature references

Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector.;Boross Peter, Breukel Cor, van Loo Pieter Fokko, van der Kaa Jos, Claassens Jill W, Bujard Hermann, Schönig Kai, Verbeek J Sjef, ;2009;Genesis (New York, N.Y. : 2000);47;729-35; 19621440

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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