B6Brd;B6N-Tyrc-Brd Prmt8tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:04479
International strain nameB6Brd;B6N-Tyrc-Brd Prmt8tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0105_1_A03
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPrmt8tm1a(EUCOMM)Wtsi
Gene/Transgene symbolPrmt8
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0105_1_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6NTac, wild-type C57BL/6NCrl
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)
IMPC phenotypes (gene matching)
MGI phenotypes (gene matching)
  • hyperactivity / MGI
  • abnormal gait / MGI
  • abnormal response to new environment / MGI
  • limb grasping / MGI
  • abnormal nervous system physiology / MGI
  • decreased neurotransmitter release / MGI
  • abnormal Purkinje cell dendrite morphology / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.;Kim Jun-Dal, Park Kyung-Eui, Ishida Junji, Kako Koichiro, Hamada Juri, Kani Shuichi, Takeuchi Miki, Namiki Kana, Fukui Hajime, Fukuhara Shigetomo, Hibi Masahiko, Kobayashi Makoto, Kanaho Yasunori, Kasuya Yoshitoshi, Mochizuki Naoki, Fukamizu Akiyoshi, ;2015;Science advances;1;e1500615; 26665171
  • Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects.;Penney Jay, Seo Jinsoo, Kritskiy Oleg, Elmsaouri Sara, Gao Fan, Pao Ping-Chieh, Su Susan C, Tsai Li-Huei, ;2017;The Journal of neuroscience : the official journal of the Society for Neuroscience;37;8655-8666; 28878098
  • Arginine Methyltransferase PRMT8 Provides Cellular Stress Tolerance in Aging Motoneurons.;Simandi Zoltan, Pajer Krisztian, Karolyi Katalin, Sieler Tatiana, Jiang Lu-Lin, Kolostyak Zsuzsanna, Sari Zsanett, Fekecs Zoltan, Pap Attila, Patsalos Andreas, Contreras Gerardo Alvarado, Reho Balint, Papp Zoltan, Guo Xiufang, Horvath Attila, Kiss Greta, Keresztessy Zsolt, Vámosi György, Hickman James, Xu Huaxi, Dormann Dorothee, Hortobagyi Tibor, Antal Miklos, Nógrádi Antal, Nagy Laszlo, ;2018;The Journal of neuroscience : the official journal of the Society for Neuroscience;38;7683-7700; 30054395

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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