B6Dnk;B6Brd;B6N-Tyrc-Brd Phf20tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:04476
International strain nameB6Dnk;B6Brd;B6N-Tyrc-Brd Phf20tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0027_2_G07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPhf20tm1a(EUCOMM)Wtsi
Gene/Transgene symbolPhf20
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0027_2_G07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • developmental and structural abnormality / IMPC
IMPC phenotypes (gene matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • developmental and structural abnormality / IMPC
MGI phenotypes (gene matching)
  • delayed bone ossification / MGI
  • fragile skeleton / MGI
  • decreased bone marrow cell number / MGI
  • small spleen / MGI
  • small thymus / MGI
  • decreased thymocyte number / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • thymus hypoplasia / MGI
  • abnormal T cell differentiation / MGI
  • abnormal hematopoietic system morphology/development / MGI
  • decreased fetal size / MGI
  • decreased lumbar vertebrae number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • intermingled spleen red and white pulp / MGI
  • decreased total body fat amount / MGI
  • neonatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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