129-Brd7^{tm2a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:04469
International strain name	129-Brd7^{tm2a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0001_3_E04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Brd7^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Brd7
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0001_3_E04. For further details on the construction of this clone see the page at the IKMC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
Breeding history	Current information may be viewed at the ^{a href="http://www.sanger.ac.uk/mouseportal/search?query=Brd7"}Sanger mouse portal^/a: Viability at weaning, Fertility, General Observations
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal behavior / IMPC
tremors / IMPC
abnormal coat/ hair morphology / IMPC
abnormal vocalization / IMPC
increased circulating glucose level / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased circulating LDL cholesterol level / IMPC
increased blood uric acid level / IMPC
decreased leukocyte cell number / IMPC
decreased body weight / IMPC

MGI phenotypes (allele matching)

decreased circulating LDL cholesterol level / MGI
abnormal coat/ hair morphology / MGI
decreased caudal vertebrae number / MGI
abnormal behavior / MGI
increased blood uric acid level / MGI
abnormal blood vessel morphology / MGI
decreased fetal size / MGI
fetal growth retardation / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
delayed limb development / MGI

MGI phenotypes (gene matching)

decreased circulating LDL cholesterol level / MGI
abnormal coat/ hair morphology / MGI
decreased caudal vertebrae number / MGI
abnormal blood vessel morphology / MGI
abnormal social/conspecific interaction / MGI
abnormal short term object recognition memory / MGI
abnormal long term object recognition memory / MGI
nervous system phenotype / MGI
decreased fetal size / MGI
fetal growth retardation / MGI
abnormal behavior / MGI
behavior/neurological phenotype / MGI
abnormal dendrite morphology / MGI
increased blood uric acid level / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
impaired spatial learning / MGI
delayed limb development / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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