B6;SJL-Tg(Mt1-SERPINE1)1Upj/Cnrm
| Status | Available to order |
| EMMA ID | EM:04468 |
| Citation information | RRID:IMSR_EM:04468 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;SJL-Tg(Mt1-SERPINE1)1Upj/Cnrm |
| Alternative name | PAI-1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Mt1-SERPINE1)1Upj |
| Gene/Transgene symbol | Tg(Mt1-SERPINE1)1Upj |
Information from provider
| Provider | Andreina Poggi |
| Provider affiliation | Consorzio Mario Negri Sud |
| Additional owner | Dr. Laurence A. Erickson and Dr. Keith R. Marotti, Pfizer (former Upjohn), Kalamazoo, MI, U.S.A. |
| Genetic information | The DNA construct used in the production of SERPINE1 (PAI-1) transgenic mice contained the metallothionein I promoter, human endothelial cell SERPINE1 complementary DNA, and the bovine growth hormone polyadenylation sequence. |
| Phenotypic information | Mice putatively transgenic for human SERPINE1 (PAI-1) showed a single, early indication of disturbance of the haemostatic system. A distinct area of subcutaneous haemorrhage was apparent at tail's tip of mice at 3 days after birth, followed by necrosis and swollen hind feet at about 12 days, as a result of venous occlusion and oedema. At about 2 weeks of age, the necrotic portion of the tail completely sloughed off, whereas the swollen hind limbs appeared normal. |
| Breeding history | SERPINE1 (PAI-1) transgenic mice originally obtained from Dr. L.A. Erickson, The Upjohn Company, Kalamazoo, Michigan USA, were backcrossed for 4 generations to C57BL/6 mice; mice were subsequently intercrossed (brother x sister) for more than 100 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Development of venous occlusions in mice transgenic for the plasminogen activator inhibitor-1 gene.;Erickson L A, Fici G J, Lund J E, Boyle T P, Polites H G, Marotti K R, ;1990;Nature;346;74-6; 2366866
- Immunohistochemical localization of fibrinolytic proteins in tissues from human PAI gene transgenic mice;Salvatore L, Iacoviello L, Bellelli E, Poggi A, Donati MB;1995;Thrombosis and Haemostasis (Abstracts);73;1005;
- A simple and reproducible model of arterial injury in normal and PAI-1 transgenic mice;Di Sciullo A, Mironov A, Nasuti M, Di Nardo P, Poggi A;1995;Thrombosis and Haemostasis (Abstracts);73;1333;