C57BL/6NTac-Wbp2^{tm2a(EUCOMM)Wtsi}/Cnrm

Status	Available to order
EMMA ID	EM:04409
International strain name	C57BL/6NTac-Wbp2^{tm2a(EUCOMM)Wtsi}/Cnrm
Alternative name	EPD0037_2_G04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Wbp2^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Wbp2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	CNR, Consiglio Nazionale delle Ricerche
Provider affiliation	EMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic information	This mouse line originates from EUCOMM ES clone EPD0037_2_G04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archiving	heterozygous C57BL/6NTac, wild-type C57BL/6NTac
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased hematocrit / IMPC
increased leukocyte cell number / IMPC
hyperactivity / IMPC
increased startle reflex / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
abnormal behavior / IMPC
absent pinna reflex / IMPC

IMPC phenotypes (gene matching)

absent pinna reflex / IMPC
decreased erythrocyte cell number / IMPC
increased startle reflex / IMPC
decreased hemoglobin content / IMPC
hyperactivity / IMPC
increased leukocyte cell number / IMPC
decreased bone mineral density / IMPC
decreased hematocrit / IMPC
abnormal behavior / IMPC

MGI phenotypes (allele matching)

abnormal organ of Corti morphology / MGI
abnormal distortion product otoacoustic emission / MGI
sensorineural hearing loss / MGI
abnormal cochlear IHC afferent innervation pattern / MGI
abnormal cochlear OHC afferent innervation pattern / MGI
hearing/vestibular/ear phenotype / MGI
abnormal auditory summating potential / MGI
decreased circulating amylase level / MGI
abnormal auditory brainstem response waveform shape / MGI
increased or absent threshold for auditory brainstem response / MGI
hyperactivity / MGI
abnormal behavior / MGI
decreased circulating serum albumin level / MGI
absent pinna reflex / MGI
decreased circulating fructosamine level / MGI

MGI phenotypes (gene matching)

abnormal organ of Corti morphology / MGI
hyperactivity / MGI
abnormal distortion product otoacoustic emission / MGI
sensorineural hearing loss / MGI
abnormal cochlear IHC afferent innervation pattern / MGI
abnormal cochlear OHC afferent innervation pattern / MGI
abnormal behavior / MGI
hearing/vestibular/ear phenotype / MGI
decreased circulating serum albumin level / MGI
absent pinna reflex / MGI
abnormal auditory summating potential / MGI
decreased circulating amylase level / MGI
decreased circulating fructosamine level / MGI
abnormal auditory brainstem response waveform shape / MGI
increased or absent threshold for auditory brainstem response / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Wbp2tm2a(EUCOMM)Wtsi/Cnrm