NMRI.Cg-Tg(CMV-cre)1Nagy/Cnbc
| Status | Available to order |
| EMMA ID | EM:04372 |
| International strain name | NMRI.Cg-Tg(CMV-cre)1Nagy/Cnbc |
| Alternative name | CMV-cre |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CMV-cre)1Nagy |
| Gene/Transgene symbol | Tg(CMV-cre)1Nagy |
Information from provider
| Provider | Andras Nagy |
| Provider affiliation | Mount Sinai Hospital, Samuel Lunenfeld Research Institute |
| Additional owner | Andras Nagy/Marina Gertsenstein are also distributing this line from CMMR, at TCP in Toronto. |
| Genetic information | General deleter Cre line. Transgene expressing Cre recombinase under the control of human CMV promoter. The transgenic line (TgN(CMV-Cre)1AN) was created by pronuclear injection of the Cre-containing HindIII fragment of the pBS185 plasmid (Sauer B, Henderson N: Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biologist 1990, 2:441-449). |
| Phenotypic information | Albino phenotype. |
| Breeding history | Initially established by Andras Nagy's laboratory using ICR mouse background, transferred to CNB-CSIC in late '90s. Since then it was backcrossed to albino outbred NMRI (>10 generations) and bred to homozygosity. Currently existing as NMRI homozygous, viable and fertile. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous NMRI, wild-type NMRI |
| Stage of embryos | 2-cell |
Literature references
- Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles.;Nagy A, Moens C, Ivanyi E, Pawling J, Gertsenstein M, Hadjantonakis A K, Pirity M, Rossant J, ;1998;Current biology : CB;8;661-4; 9635194