NMRI.Cg-Tg(CMV-cre)1Nagy/Cnbc

Status	Available to order
EMMA ID	EM:04372
Citation information	RRID:IMSR_EM:04372 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	NMRI.Cg-Tg(CMV-cre)1Nagy/Cnbc
Alternative name	CMV-cre
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CMV-cre)1Nagy
Gene/Transgene symbol	Tg(CMV-cre)1Nagy

Information from provider

Provider	Andras Nagy
Provider affiliation	Mount Sinai Hospital, Samuel Lunenfeld Research Institute
Additional owner	Andras Nagy/Marina Gertsenstein are also distributing this line from CMMR, at TCP in Toronto.
Genetic information	General deleter Cre line. Transgene expressing Cre recombinase under the control of human CMV promoter. The transgenic line (TgN(CMV-Cre)1AN) was created by pronuclear injection of the Cre-containing HindIII fragment of the pBS185 plasmid (Sauer B, Henderson N: Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biologist 1990, 2:441-449).
Phenotypic information	Albino phenotype.
Breeding history	Initially established by Andras Nagy's laboratory using ICR mouse background, transferred to CNB-CSIC in late '90s. Since then it was backcrossed to albino outbred NMRI (>10 generations) and bred to homozygosity. Currently existing as NMRI homozygous, viable and fertile.
References	Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles.;Nagy A, Moens C, Ivanyi E, Pawling J, Gertsenstein M, Hadjantonakis A K, Pirity M, Rossant J, ;1998;Current biology : CB;8;661-4; 9635194
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous NMRI males, wild-type NMRI females
Stage of embryos	2-cell

Literature references

Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles.;Nagy A, Moens C, Ivanyi E, Pawling J, Gertsenstein M, Hadjantonakis A K, Pirity M, Rossant J, ;1998;Current biology : CB;8;661-4; 9635194

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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