| Status | Available to order |
| EMMA ID | EM:04326 |
| International strain name | STOCK Vezttm1.1Smc/Vezttm1.2Smc/Orl |
| Alternative name | Vezatin flox5/null (or bi-loxP/null vezt strain) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Vezttm1.1Smc, Vezttm1.2Smc |
| Gene/Transgene symbol | Vezt, Vezt |
| Provider | Marie-Christine SIMMLER |
| Provider affiliation | Neurobiology and development, CNRS |
| Genetic information | Vezatin is a integral membrane protein associated to E-cadherin adherens junctions. Exon 5 encodes the transmembranous domain. A conditional vezatin allele lacking exon 5 was generated by using a cre/loxP strategy (original vezt floxed line, EM:01817). This is an heterozygous line modified as follows: one chromosome is carrying an exon-5-only-floxed (Vezt flox5) vezatin allele, i.e. deleted of the floxed pPgk-1-neo-pA cassette (by partial cre recombinase activity) and one chromosome is carrying a null (defloxed) vezatin allele (after deletion of the exon5+neo floxed segment using the pgk1-cre). |
| Phenotypic information | Ubiquitous targeted disruption of vezatin results in embryonic lethality due to a failure of epithelialization of the trophectoderm (Hyenne et al., 2007). Hairs cells targeted disruption results in noise-induced hearing loss (Balhoul et al., 2009). |
| Breeding history | More than N10 breeding on a mixed/randomized 129/SvPas x B6N. Breeding procedures of heterozygous Vezt Flox5/null x Vezt Flox5/null will only produce Vezt Flox5/null (66%) and Vezt Flox5/Flox5 (33%) mice, since Vezt null/null is an embryonic lethal condition. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129/Sv x C57BL/6N, heterozygous 129/Sv x C57BL/6N |
