| Status | Available to order |
| EMMA ID | EM:04326 |
| Citation information | RRID:IMSR_EM:04326 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Vezttm1.1Smc/Vezttm1.2Smc/Orl |
| Alternative name | Vezatin flox5/null (or bi-loxP/null vezt strain) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Vezttm1.1Smc, Vezttm1.2Smc |
| Gene/Transgene symbol | Vezt, Vezt |
| Provider | Marie-Christine SIMMLER |
| Provider affiliation | Neurobiology and development, CNRS |
| Genetic information | Vezatin is a integral membrane protein associated to E-cadherin adherens junctions. Exon 5 encodes the transmembranous domain. A conditional vezatin allele lacking exon 5 was generated by using a cre/loxP strategy (original vezt floxed line, EM:01817). This is an heterozygous line modified as follows: one chromosome is carrying an exon-5-only-floxed (Vezt flox5) vezatin allele, i.e. deleted of the floxed pPgk-1-neo-pA cassette (by partial cre recombinase activity) and one chromosome is carrying a null (defloxed) vezatin allele (after deletion of the exon5+neo floxed segment using the pgk1-cre). |
| Phenotypic information | Ubiquitous targeted disruption of vezatin results in embryonic lethality due to a failure of epithelialization of the trophectoderm (Hyenne et al., 2007). Hairs cells targeted disruption results in noise-induced hearing loss (Balhoul et al., 2009). |
| Breeding history | More than N10 breeding on a mixed/randomized 129/SvPas x B6N. Breeding procedures of heterozygous Vezt Flox5/null x Vezt Flox5/null will only produce Vezt Flox5/null (66%) and Vezt Flox5/Flox5 (33%) mice, since Vezt null/null is an embryonic lethal condition. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129/Sv x C57BL/6N males, heterozygous 129/Sv x C57BL/6N females |
