B6Dnk;B6N-Smpdl3btm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:04270
Citation informationRRID:IMSR_EM:04270 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6Dnk;B6N-Smpdl3btm1a(EUCOMM)Wtsi/H
Alternative nameEPD0060_2_A11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSmpdl3btm1a(EUCOMM)Wtsi
Gene/Transgene symbolSmpdl3b
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0060_2_A11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivinghomozygous C57BL/6Dnk males
Breeding at archiving centreChimeras were backcrossed to C57BL/6N Tac Den, and maintained on this background thereafter.

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased neutrophil cell number / MGI
  • abnormal macrophage morphology / MGI
  • increased circulating tumor necrosis factor level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-12b level / MGI
  • abnormal chemokine secretion / MGI
  • increased susceptibility to endotoxin shock / MGI
  • abnormal circulating chemokine level / MGI
MGI phenotypes (gene matching)
  • increased neutrophil cell number / MGI
  • abnormal macrophage morphology / MGI
  • increased circulating tumor necrosis factor level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-12b level / MGI
  • abnormal chemokine secretion / MGI
  • increased susceptibility to endotoxin shock / MGI
  • abnormal circulating chemokine level / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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