B6Dnk;B6N-Elk4^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:04263
Citation information	RRID:IMSR_EM:04263 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Dnk;B6N-Elk4^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0100_4_A06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Elk4^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Elk4
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0100_4_A06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6Dnk males
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac Den and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)

increased bone mineral density / IMPC
decreased bone mineral density / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased circulating HDL cholesterol level / IMPC
abnormal cornea morphology / IMPC
corneal opacity / IMPC
abnormal retina morphology / IMPC
decreased circulating triglyceride level / IMPC
decreased erythrocyte cell number / IMPC
increased lactate dehydrogenase level / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating glycerol level / IMPC
abnormal bone structure / IMPC
decreased lean body mass / IMPC
increased B cell number / IMPC
increased T cell number / IMPC
decreased T cell number / IMPC
decreased circulating cholesterol level / IMPC
increased circulating aspartate transaminase level / IMPC
decreased circulating serum albumin level / IMPC
increased circulating sodium level / IMPC
decreased circulating sodium level / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased mature B cell number / IMPC
increased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased B cell number / IMPC
abnormal retina morphology / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased circulating triglyceride level / IMPC
decreased circulating cholesterol level / IMPC
decreased T cell number / IMPC
increased circulating sodium level / IMPC
decreased circulating glycerol level / IMPC
increased T cell number / IMPC
corneal opacity / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
increased circulating aspartate transaminase level / IMPC
preweaning lethality, complete penetrance / IMPC
decreased erythrocyte cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased circulating serum albumin level / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased mature B cell number / IMPC
increased bone mineral content / IMPC
decreased bone mineral density / IMPC
abnormal cornea morphology / IMPC
decreased circulating HDL cholesterol level / IMPC
increased bone mineral density / IMPC
decreased circulating sodium level / IMPC
abnormal bone structure / IMPC
decreased lean body mass / IMPC
increased lactate dehydrogenase level / IMPC

MGI phenotypes (gene matching)

decreased thymocyte number / MGI
abnormal T cell selection / MGI
abnormal positive T cell selection / MGI
decreased T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
decreased single-positive T cell number / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6N-Elk4tm1a(EUCOMM)Wtsi/H