B6Dnk;B6N-Brpf1tm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:04257
Citation informationRRID:IMSR_EM:04257 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6Dnk;B6N-Brpf1tm1a(EUCOMM)Wtsi/H
Alternative nameEPD0069_1_G06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolBrpf1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolBrpf1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0069_1_G06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6Dnk males
Breeding at archiving centreChimeras were mated to C57BL/6N Tac Den mice and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased bone mineral density / IMPC
  • increased startle reflex / IMPC
IMPC phenotypes (gene matching)
  • decreased bone mineral density / IMPC
  • increased startle reflex / IMPC
MGI phenotypes (allele matching)
  • embryonic lethality, complete penetrance / MGI
MGI phenotypes (gene matching)
  • embryonic growth arrest / MGI
  • no abnormal phenotype detected / MGI
  • abnormal cell physiology / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • abnormal embryo morphology / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

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