B6Dnk;B6N-Slc38a10^{tm2a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:04231
International strain name	B6Dnk;B6N-Slc38a10^{tm2a(EUCOMM)Wtsi}/H
Alternative name	EPD0023_1_F07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Slc38a10^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Slc38a10
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0023_1_F07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6Dnk
Breeding at archiving centre	Chimeras mated to C57BL/6N Tac Den and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased circulating calcium level / IMPC
decreased body length / IMPC
abnormal retina morphology / IMPC
abnormal gait / IMPC
abnormal startle reflex / IMPC
increased circulating phosphate level / IMPC
abnormal eye morphology / IMPC
increased mean corpuscular volume / IMPC
decreased circulating triglyceride level / IMPC
short tibia / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
decreased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
decreased lean body mass / IMPC
abnormal response to tactile stimuli / IMPC
increased circulating aspartate transaminase level / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating total protein level / IMPC
decreased circulating sodium level / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
decreased circulating amylase level / IMPC
decreased grip strength / IMPC
increased bone mineral content / IMPC

IMPC phenotypes (gene matching)

lipid deposition / IMPC
decreased body length / IMPC
decreased body weight / IMPC
abnormal cranium morphology / IMPC
lipid depletion / IMPC
increased mean corpuscular volume / IMPC
short tibia / IMPC
decreased circulating serum albumin level / IMPC
increased bone mineral content / IMPC
abnormal bone structure / IMPC
abnormal gait / IMPC
decreased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
decreased circulating triglyceride level / IMPC
abnormal response to tactile stimuli / IMPC
decreased total body fat amount / IMPC
abnormal startle reflex / IMPC
decreased lean body mass / IMPC
decreased circulating calcium level / IMPC
decreased circulating sodium level / IMPC
decreased hemoglobin content / IMPC
abnormal eye morphology / IMPC
decreased circulating amylase level / IMPC
decreased erythrocyte cell number / IMPC
decreased bone mineral content / IMPC
increased circulating aspartate transaminase level / IMPC
increased circulating phosphate level / IMPC
abnormal retina morphology / IMPC
decreased circulating total protein level / IMPC
decreased grip strength / IMPC
abnormal maxilla morphology / IMPC
decreased bone mineral density / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
increased circulating iron level / IMPC

MGI phenotypes (gene matching)

fragile skeleton / MGI
decreased bone mineral density / MGI
decreased circulating calcium level / MGI
abnormal cranium morphology / MGI
abnormal maxilla morphology / MGI
abnormal liver physiology / MGI
decreased body length / MGI
decreased body weight / MGI
decreased hemoglobin content / MGI
decreased erythrocyte cell number / MGI
decreased circulating alkaline phosphatase level / MGI
decreased lean body mass / MGI
decreased length of long bones / MGI
increased energy expenditure / MGI
increased oxygen consumption / MGI
decreased circulating serum albumin level / MGI
increased circulating creatinine level / MGI
decreased circulating total protein level / MGI
decreased circulating amylase level / MGI
decreased total body fat amount / MGI
decreased bone mineral content / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6N-Slc38a10tm2a(EUCOMM)Wtsi/H