B6;129P2-Tcratm1Mjo/Orl

Status

Available to order

EMMA IDEM:00041
Citation informationRRID:IMSR_EM:00041 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6;129P2-Tcratm1Mjo/Orl
Alternative nameCalpha
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTcratm1Mjo
Gene/Transgene symbolTcra

Information from provider

ProviderKaren Philpott
Provider affiliationImperial Cancer Research Fund
Genetic informationTargeting construct is derived from 129 DNA. The deletion spans all the repeats known in the Emu-Cmu intron leaving intact the necessary elements for accurate splicing of mu transcripts. The homozygous mutant mice are generated by a targeted mutation of the TCR alpha gene, by insertion of a neomycin-resistance cassette into the exon coding for the extracellular C-alpha domain, via homologous recombination.
Phenotypic informationThe homozygous animals show elimination of alpha beta+ cells and the absence of thymic medullae. In contrast, they present a normal number of gamma delta+ T cells.
References
  • Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells.;Philpott K L, Viney J L, Kay G, Rastan S, Gardiner E M, Chae S, Hayday A C, Owen M J, ;1992;Science (New York, N.Y.);256;1448-52; 1604321

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal T cell physiology / MGI
  • decreased susceptibility to autoimmune diabetes / MGI
  • immune system phenotype / MGI
  • increased activated T cell number / MGI
  • rectal prolapse / MGI
  • abnormal colon morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • colitis / MGI
  • increased T cell number / MGI
  • diarrhea / MGI
  • decreased T cell proliferation / MGI
  • increased gamma-delta T cell number / MGI
  • enlarged mesenteric lymph nodes / MGI
  • abnormal neuron proliferation / MGI
  • abnormal thymus cortex morphology / MGI
  • increased immunoglobulin level / MGI
  • increased IgE level / MGI
  • abnormal immune system organ morphology / MGI
  • increased autoantibody level / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • increased B cell number / MGI
  • abnormal T cell morphology / MGI
  • small Peyer's patches / MGI
  • increased IgG1 level / MGI
  • abnormal splenic cell ratio / MGI
  • absent thymus medulla / MGI
  • enlarged thymus cortex / MGI
  • abnormal kidney morphology / MGI
  • increased urine protein level / MGI
MGI phenotypes (gene matching)
  • abnormal large intestine morphology / MGI
  • crypts of Lieberkuhn abscesses / MGI
  • rectal prolapse / MGI
  • abnormal colon morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • intestinal ulcer / MGI
  • abnormal liver morphology / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • decreased thymocyte number / MGI
  • chronic diarrhea / MGI
  • decreased IgG level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased activated T cell number / MGI
  • premature death / MGI
  • abnormal respiratory system morphology / MGI
  • abnormal kidney morphology / MGI
  • abnormal T cell differentiation / MGI
  • abnormal thymus cortex morphology / MGI
  • abnormal double-negative T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal T cell physiology / MGI
  • increased immunoglobulin level / MGI
  • increased IgE level / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • increased urine protein level / MGI
  • abnormal cytokine secretion / MGI
  • no phenotypic analysis / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • increased autoantibody level / MGI
  • abnormal T cell subpopulation ratio / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • decreased susceptibility to autoimmune diabetes / MGI
  • increased B cell number / MGI
  • increased T cell number / MGI
  • abnormal response to infection / MGI
  • diarrhea / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • immune system phenotype / MGI
  • abnormal response to transplant / MGI
  • abnormal NK T cell morphology / MGI
  • abnormal T cell morphology / MGI
  • increased NK T cell number / MGI
  • absent T cells / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • small Peyer's patches / MGI
  • increased gamma-delta T cell number / MGI
  • increased gamma-delta intraepithelial T cell number / MGI
  • increased mature gamma-delta T cell number / MGI
  • enlarged mesenteric lymph nodes / MGI
  • decreased IgG2a level / MGI
  • increased IgG1 level / MGI
  • increased interferon-gamma secretion / MGI
  • increased interleukin-10 secretion / MGI
  • increased interleukin-2 secretion / MGI
  • increased interleukin-4 secretion / MGI
  • abnormal T cell receptor alpha chain V-J recombination / MGI
  • abnormal splenic cell ratio / MGI
  • decreased splenocyte number / MGI
  • abnormal neuron proliferation / MGI
  • absent thymus medulla / MGI
  • enlarged thymus cortex / MGI

Literature references

  • Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells.;Philpott K L, Viney J L, Kay G, Rastan S, Gardiner E M, Chae S, Hayday A C, Owen M J, ;1992;Science (New York, N.Y.);256;1448-52; 1604321

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

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Legally binding conditions for the transfer

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