B6Dnk;B6N-Aldh2^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:04096
International strain name	B6Dnk;B6N-Aldh2^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0089_4_F11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Aldh2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Aldh2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0089_4_F11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6Dnk, wild-type C57BL/6Dnk
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased hematocrit / IMPC
abnormal startle reflex / IMPC
increased IgE level / IMPC
decreased erythrocyte cell number / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased circulating glycerol level / IMPC
decreased T cell number / IMPC
decreased circulating cholesterol level / IMPC
abnormal response to tactile stimuli / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
increased IgG1 level / IMPC
increased bone mineral content / IMPC

IMPC phenotypes (gene matching)

abnormal lens morphology / IMPC
increased bone mineral content / IMPC
abnormal startle reflex / IMPC
increased IgG1 level / IMPC
decreased T cell number / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased erythrocyte cell number / IMPC
abnormal response to tactile stimuli / IMPC
decreased circulating cholesterol level / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased hematocrit / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased circulating glycerol level / IMPC
increased IgE level / IMPC
decreased bone mineral density / IMPC

MGI phenotypes (allele matching)

decreased litter size / MGI

MGI phenotypes (gene matching)

decreased leukocyte cell number / MGI
hyperpigmentation / MGI
decreased body weight / MGI
cardiac hypertrophy / MGI
abnormal cardiac output / MGI
abnormal heart rate / MGI
decreased litter size / MGI
abnormal nociception after inflammation / MGI
decreased alcohol consumption / MGI
increased hepatocyte apoptosis / MGI
increased incidence of tumors by chemical induction / MGI
decreased cardiac muscle contractility / MGI
hyperalgesia / MGI
abnormal circulating hormone level / MGI
abnormal ethanol metabolism / MGI
abnormal enzyme/coenzyme activity / MGI
congestive heart failure / MGI
enhanced behavioral response to alcohol / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased tumor latency / MGI
abnormal cellular respiration / MGI
impaired exercise endurance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6N-Aldh2tm1a(EUCOMM)Wtsi/Ieg