B6;FVB-Tg(GFP/KRAS2/ALPP)1Brn/Cnrm

Status	Available to order
EMMA ID	EM:00409
Citation information	RRID:IMSR_EM:00409 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;FVB-Tg(GFP/KRAS2/ALPP)1Brn/Cnrm
Alternative name	RasF
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(GFP/KRAS2/ALPP)1Brn
Gene/Transgene symbol	Tg(GFP/KRAS2/ALPP)1Brn

Information from provider

Provider	Anton Berns
Provider affiliation	Research - Animal Facility, The Netherlands Cancer Institute
Genetic information	Zygote injected transgene: chicken actin promoter-loxP-GFP-bGHpolyA-loxP-Kras^v12-IRES-hPLAP. A 1.2 kb human cDNA fragment encoding K-ras^v12 was placed under the control of the 1.8 kb chicken beta-actin promoter. In between the promoter and K-ras^v12 a 1.3 kb GFP cassette with a poly-A linker was placed, flanked by two loxP sites. This GFP cassette served as a marker for beta-actin promoter activity. Behind the K-Ras^v12 a 2.9 kb human placenta-like alkaline phosphatase (ALPP or PLAP) was placed as a reporter of K-Ras^v12 expression.
Phenotypic information	Conditional K-ras^v12 transgene, females might develop mammary tumors.
Breeding history	Currently backcrossed to C57BL/6.
References	Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene.;Meuwissen R, Linn S C, van der Valk M, Mooi W J, Berns A, ;2001;Oncogene;20;6551-8; 11641780

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene.;Meuwissen R, Linn S C, van der Valk M, Mooi W J, Berns A, ;2001;Oncogene;20;6551-8; 11641780

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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