B6Dnk;B6N-PmlGt(EUCJ0019c12)Hmgu/Ieg

Status

Available to order

EMMA IDEM:04086
International strain nameB6Dnk;B6N-PmlGt(EUCJ0019c12)Hmgu/Ieg
Alternative nameEUCJ0019c12
Strain typeGene-trap
Allele/Transgene symbolPmlGt(EUCJ0019c12)Hmgu
Gene/Transgene symbolPml
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EUCJ0019c12. For further details on the construction of this clone see the page at HTGT. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele. Click here for more information on EUCOMM final vectors.
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6Dnk, wild-type C57BL/6Dnk
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased circulating alkaline phosphatase level / IMPC
IMPC phenotypes (gene matching)
  • increased circulating alkaline phosphatase level / IMPC
MGI phenotypes (allele matching)
  • no phenotypic analysis / MGI
MGI phenotypes (gene matching)
  • decreased leukocyte cell number / MGI
  • decreased granulocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • abnormal myelopoiesis / MGI
  • neoplasm / MGI
  • increased tumor incidence / MGI
  • increased B cell derived lymphoma incidence / MGI
  • increased T cell derived lymphoma incidence / MGI
  • no abnormal phenotype detected / MGI
  • increased susceptibility to bacterial infection / MGI
  • no phenotypic analysis / MGI
  • elevated level of mitotic sister chromatid exchange / MGI
  • increased cellular sensitivity to ionizing radiation / MGI
  • increased hematopoietic stem cell number / MGI
  • abnormal cell physiology / MGI
  • abnormal PML bodies / MGI
  • abnormal double-strand DNA break repair / MGI
  • increased fibroblast proliferation / MGI
  • increased lymphoma incidence / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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