B6Dnk;B6N-Uhrf1^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:04084
International strain name	B6Dnk;B6N-Uhrf1^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0052_1_G08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Uhrf1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Uhrf1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0052_1_G08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacDen, wild-type C57BL/6NTacDen
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased body weight / IMPC
abnormal gait / IMPC
abnormal eye morphology / IMPC
decreased mean corpuscular volume / IMPC
abnormal retina pigmentation / IMPC
increased circulating aspartate transaminase level / IMPC
increased NK cell number / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased NK cell number / IMPC
increased circulating aspartate transaminase level / IMPC
abnormal retina pigmentation / IMPC
abnormal gait / IMPC
preweaning lethality, complete penetrance / IMPC
decreased mean corpuscular volume / IMPC
abnormal eye morphology / IMPC
decreased body weight / IMPC

MGI phenotypes (gene matching)

abnormal mesoderm development / MGI
abnormal allantois morphology / MGI
embryonic growth arrest / MGI
abnormal nervous system morphology / MGI
abnormal neural tube closure / MGI
embryonic growth retardation / MGI
abnormal sclerotome morphology / MGI
small pharyngeal arch / MGI
abnormal DNA methylation / MGI
embryonic lethality during organogenesis, complete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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